Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HT29 cell line (colon cancer epithelial cell line))
Permeabilization
Yes - Tween 0,25%
Specification
HT29 cell line (colon cancer epithelial cell line)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Methanol
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/50
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor anti-rabbit 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 30 2015