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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor

Anti-Cannabinoid Receptor II抗体(ab3561)

  • Datasheet
  • SDS
Reviews (17)Q&A (15)References (53)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)

Key features and details

  • Rabbit polyclonal to Cannabinoid Receptor II
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG

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概述

  • 产品名称

    Anti-Cannabinoid Receptor II抗体
    参阅全部 Cannabinoid Receptor II 一抗
  • 描述

    兔多克隆抗体to Cannabinoid Receptor II
  • 宿主

    Rabbit
  • 特异性

    We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    Recombinant fragment
    WB
    Human
    See all applications and species data
  • 免疫原

    Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).
    Database link: Q9QZN9

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • Primary antibody说明

    Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Cannabinoid Receptor

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cannabinoid Receptor II protein (ab114225)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
Recombinant fragment
WB
Human
应用 Ab评论 说明
ICC
Use at an assay dependent concentration.
WB (4)
1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P (2)
1/10 - 1/100.
说明
ICC
Use at an assay dependent concentration.
WB
1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P
1/10 - 1/100.

靶标

  • 功能

    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • 组织特异性

    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family.
  • 翻译后修饰

    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • 细胞定位

    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Target information above from: UniProt accession P34972 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 1269 Human
    • Entrez Gene: 57302 Rat
    • Omim: 605051 Human
    • SwissProt: P34972 Human
    • SwissProt: Q9QZN9 Rat
    • Unigene: 73037 Human
    • Unigene: 48776 Rat
    • 别名

      • Cannabinoid receptor 2 antibody
      • Cannabinoid receptor 2 macrophage antibody
      • CB 2 antibody
      • CB-2 antibody
      • CB2 antibody
      • CNR2 antibody
      • CNR2_HUMAN antibody
      • CNRII antibody
      • CX 5 antibody
      • CX5 antibody
      • hCB2 antibody
      • testis-dominant CNR2 isoform CB2 antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)

      ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)

      ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
      Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
      All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution

      Lane 1 : HT29 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Rat colon cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)

      Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (53)

    发表研究结果有使用 ab3561?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3561 被引用在 53 文献中.

    • Remiszewski P  et al. Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32075117
    • Gupta B  et al. Anti-apoptotic and Immunomodulatory Effect of CB2 Agonist, JWH133, in a Neonatal Rat Model of Hypoxic-Ischemic Encephalopathy. Front Pediatr 8:65 (2020). PubMed: 32175293
    • Huang W  et al. NOX2 is involved in CB2-mediated protection against lung ischemia-reperfusion injury in mice. Int J Clin Exp Pathol 13:277-285 (2020). PubMed: 32211110
    • Jing N  et al. Exogenous activation of cannabinoid-2 receptor modulates TLR4/MMP9 expression in a spinal cord ischemia reperfusion rat model. J Neuroinflammation 17:101 (2020). PubMed: 32248810
    • Baranowska-Kuczko M  et al. Vasodilatory effects of cannabidiol in human pulmonary and rat small mesenteric arteries: modification by hypertension and the potential pharmacological opportunities. J Hypertens 38:896-911 (2020). PubMed: 31800399
    View all Publications for this product

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    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Cannabinoid Receptor II antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (bladder)
    Loading amount
    100 µg
    Specification
    bladder
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More

    Dr. Eun-Young Park

    Verified customer

    提交于 Apr 09 2013

    Western blot abreview for Anti-Cannabinoid Receptor II antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Recombinant protein (Brian)
    Loading amount
    10 µg
    Specification
    Brian
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 01 2011

    Western blot abreview for Anti-Cannabinoid Receptor II antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary cortical microglia)
    Loading amount
    100000 cells
    Specification
    primary cortical microglia
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Blocking step
    Protein-free Blocking Solution (Pierce) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jan 18 2010

    Western blot abreview for Anti-Cannabinoid Receptor II antibody

    Poor
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (DLD-1 cells)
    Loading amount
    40000 cells
    Specification
    DLD-1 cells
    Gel Running Conditions
    Reduced Denaturing (4-12% gel)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 28 2009

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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