Anti-Cannabinoid Receptor II抗体(ab3561)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor II
- Suitable for: ICC, WB, IHC-P
- Reacts with: Rat, Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Cannabinoid Receptor II抗体
参阅全部 Cannabinoid Receptor II 一抗 -
描述
兔多克隆抗体to Cannabinoid Receptor II -
宿主
Rabbit -
特异性
We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.
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Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanRecombinant fragmentWB Human
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免疫原
Fusion protein corresponding to Rat Cannabinoid Receptor II aa 1-32 (N terminal).
Database link: Q9QZN9 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 0.1% BSA, 49% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| 应用 | Species |
|---|---|
| IHC-P |
Human
Recombinant fragment
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| WB |
Human
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| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
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| WB | (4) |
1/50 - 1/500. Predicted molecular weight: 40 kDa.
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| IHC-P | (2) |
1/10 - 1/100.
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| 说明 |
|---|
|
ICC
Use at an assay dependent concentration. |
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WB
1/50 - 1/500. Predicted molecular weight: 40 kDa. |
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IHC-P
1/10 - 1/100. |
靶标
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功能
Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. -
组织特异性
Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level). -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
翻译后修饰
Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor. -
细胞定位
Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons. - Information by UniProt
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数据库链接
- Entrez Gene: 1269 Human
- Entrez Gene: 57302 Rat
- Omim: 605051 Human
- SwissProt: P34972 Human
- SwissProt: Q9QZN9 Rat
- Unigene: 73037 Human
- Unigene: 48776 Rat
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别名
- Cannabinoid receptor 2 antibody
- Cannabinoid receptor 2 macrophage antibody
- CB 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution
Lane 1 : HT29 cell lysate
Lane 2 : C6 cell lysate
Lane 3 : Rat colon cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (53)
ab3561 被引用在 53 文献中.
- Remiszewski P et al. Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32075117
- Gupta B et al. Anti-apoptotic and Immunomodulatory Effect of CB2 Agonist, JWH133, in a Neonatal Rat Model of Hypoxic-Ischemic Encephalopathy. Front Pediatr 8:65 (2020). PubMed: 32175293
- Huang W et al. NOX2 is involved in CB2-mediated protection against lung ischemia-reperfusion injury in mice. Int J Clin Exp Pathol 13:277-285 (2020). PubMed: 32211110
- Jing N et al. Exogenous activation of cannabinoid-2 receptor modulates TLR4/MMP9 expression in a spinal cord ischemia reperfusion rat model. J Neuroinflammation 17:101 (2020). PubMed: 32248810
- Baranowska-Kuczko M et al. Vasodilatory effects of cannabidiol in human pulmonary and rat small mesenteric arteries: modification by hypertension and the potential pharmacological opportunities. J Hypertens 38:896-911 (2020). PubMed: 31800399