Anti-Cannabinoid Receptor II抗体(ab3561)

Thank you for that clarification.

I have sent you a FedEx link that you can complete to be able to return the vial of ab3561 from order number *. Once that order is returned to us, we will credit the cost of that product back to you. The credit note for this is at the bottom of the email.

As for the vial that you recieved on order number *, as we discussed I am sending you a vial of ab3560. The order number for this is 1* and you should have it on Tueday morning.

If there is anything else I can help you with, please let me know.

Credit Note for order #*

Your credit note ID is *.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Thank you for that information.

That order number (*), is for the vial of ab3561 that you just received today, correct? If so, I will go ahead and set up the return and credit for this order.

Do you have the order number for the first vial of ab3561 that you received, as I will need that number to send the free of charge replacement.

Sorry, I do not mean to make this complicated; I just want to make sure I replace/refund the correct orders.

Thank you for your patience and cooperation and I hope to have this resolved quickly.

Thank you for your reply.

I am sorry about the frustration you are having with ab3561 in flow cytometry and I would be happy to replace the first vial that you received. Of the two possible replacements you mentioned in you previous email, we only guarantee ab3560 to work in flow cytometry, although we have only tested it in rat and human samples. I know you were transfecting CHO cells, but is the construct for rat or human?

For the vial of ab3561 that you just received, if you could please provide the order number for this product, I will make arrangements for the cost to be credited back to your account and also for you to return that vial to Abcam.

I look forward to your reply and helping to resolve this issue.

Thank you for contacting Abcam.

The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on a number of samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

The information we have regarding concentrations for the antibody working in flow cytometry, come from some references, which are available on the website. That information is also below:

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20206138&dopt=Abstract - They used 1/100 dilution.
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=18239598&dopt=Abstract - They do not specify the concentration they used.



If you cannot find any protocol information in these references that prove to be useful, then please feel free to send me a copy of the protocol and results that you are getting and I would be happy to look them over and see if there is anything that I can suggest.

Please let me know if there is anything else I can help you with.

Thank you for contacting us.

The number of slides that could be used with this kit depends on the tissue sections, and the volume of reagents per slide. The volume used should be enough to cover them. We normally use 200ul per slide in house, but 100ul should be enough. If 100ul are used then you could run 150 slides with the reagents provided.

This kit is compatible with any primary antibody suitable for IHC in paraffin sections, as long as the recommendations and protocols are followed.

Regarding the tissue slides to use as controls, I checked the http://www.proteinatlas.org/ENSG00000188822, which suggests digestive tract, female tissues or kidney as positive controls. Other tissues could be Human Spinal Cord, Rat Spinal Cord, and Mouse Thymus. I can check if you are interested what would be a good negative control with my lab colleagues.

If you are using the EXPOSE kit there is no need to use an enhancer for detection.

Except for the products ab94726 and ab64026, the rest of them are currently in stock, and you will receive them within two working days.

Please note all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

You will receive the proforma invoice with the requested products shortly.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you very much for your reply and for sending the additional information.

Your credit note ID is ***

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department at mailto:us.credits@abcam.com so that we may gather the correct information needed for the refund. Please refer to the credit note ID in any correspondence with our accounting department.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further assistance or advice.

Thank you very much for your call today and for letting us know about the trouble with this antibody.

If you could please provide the following information I will arrange the refund promptly:

1) What kind of mouse samples were used (tissue or cell type)?
2) What application was the antibody used in?
3) Could you describe the results that were seen?
4) How were the samples prepared or fixed?
5) What kind of blocking solution was used?
6) At what dilution was the antibody used?


I look forward to hearing from you. Please let me know if you have any further questions or if there is anything else that we can do for you.