参阅全部 CaMKII beta 一抗
描述兔多克隆抗体to CaMKII beta
特异性This antibody does cross-react with CAMKII alpha protein
经测试应用适用于: IHC-FoFr, ICC/IF, WB, IHC-Pmore details
种属反应性与反应: Mouse, Rat
- This antibody gave a positive signal in the following lysates: Brain (Mouse) Tissue Brain (Rat) Tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: PC12.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
纯度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab34703 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).Can be blocked with Mouse CaMKII beta peptide (ab27843).|
|IHC-P||Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
功能CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses, it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity.
组织特异性Widely expressed. Expressed in adult and fetal brain. Expression is slightly lower in fetal brain.
序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
形式The multifunctional CaMKII beta, or Ca2+/calmodulin-dependent protein kinase II, is a well known effector of calcium- and calmodulin- mediated functions. It is present in many tissues but is most abundant in the brain. CaMKII is composed of four different chains: alpha, beta, gamma, and delta. The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits.
- Calcium/calmodulin dependent protein kinase (CaM kinase) II beta antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase IIB antibody
All lanes : Anti-CaMKII beta antibody (ab34703) at 1 µg/ml
Lane 1 : Brain Mouse Tissue Lysate
Lane 2 : Brain Rat Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDa
ab34703 staining CaMKII in rat brain tissue section (hippocampus) by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue from 4% PFA perfused animals underwent overnight fixation in 4% paraformaldehyde, cryoprotected in 30% sucrose and cut using cryostat.The primary antibody was diluted, 1/300 (PBS + 0.3% Triton X100) and incubated with sample for 18 hours at 20°C. An abcam antibody ab60314, Chromeo488 conjugated goat polyclonal to rabbit IgGwas used as secondary at 1/1000 dilution.
ab34703 staining CaMKII beta in adult mouse cerebellum tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with paraformaldehyde and a heat mediated antigen retrieval step was performed using 0.01M Tris HCL, pH 10. Samples were then permeabilized using 0.3% Triton X-100, blocked with 5% BSA for 45 minutes at 22°C and then incubated with ab34703 at a 1/3000 dilution for 16 hours at 4°C. The secondary used was a biotin conjugated horse polyclonal used at a 1/2000 dilution.
Strong siganls were detected in molecular layers (M), and Purkinje cells (P) were positively stained as well. In granule layers (G), some neurons were also strongly stained.
ICC/IF image of ab34703 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab34703 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This product has been referenced in:
- Zhou M et al. Quantitative Proteomic Analysis Reveals Synaptic Dysfunction in the Amygdala of Rats Susceptible to Chronic Mild Stress. Neuroscience 376:24-39 (2018). WB . Read more (PubMed: 29452226) »
- Incontro S et al. The CaMKII/NMDA receptor complex controls hippocampal synaptic transmission by kinase-dependent and independent mechanisms. Nat Commun 9:2069 (2018). Read more (PubMed: 29802289) »