重组Anti-CaMKII抗体[EPR6685(B)] (ab126789)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6685(B)] to CaMKII
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-CaMKII抗体[EPR6685(B)]
参阅全部 CaMKII 一抗 -
描述
兔单克隆抗体[EPR6685(B)] to CaMKII -
宿主
Rabbit -
特异性
The peptide immunogen shares a 100% homology with CaMKII alpha, gamma and delta therefore we believe cross-reactivity is highly likely but this has not been experimentally assessed.
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经测试应用
适用于: Flow Cyt (Intra), WBmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性
与反应: Rat, Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human pancreas, fetal kidney, HeLa, Jurkat, SH SY5Y, BxPC3 and C6 lysates; Permeabilized C6 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解离常数(KD)
KD = 2.60 x 10 -11 M Learn more about KD -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR6685(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126789于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 51-73 kDa).
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说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 45-70 kDa (predicted molecular weight: 51-73 kDa). |
靶标
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功能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
序列相似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
细胞定位
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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数据库链接
- Entrez Gene: 815 Human
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12322 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
see all -
别名
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
图片
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All lanes : Anti-CaMKII antibody [EPR6685(B)] (ab126789) at 1/1000 dilution
Lane 1 : Human pancreas lysate
Lane 2 : Human fetal kidney tissue lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat lysate
Lane 5 : SH-SY5Y cell lysate
Lane 6 : BxPC-3 cell lysate
Lane 7 : C6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 51-73 kDa -
ab126789 at 1/10 dilution staining CaMKII in permeabilized C6 cells by intracellular flow cytometry (red) compared with a rabbit IgG negative control (green).
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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