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Signal Transduction Signaling Pathway Calcium Signaling Calcium Binding Proteins

Anti-Calsequestrin抗体(ab3516)

  • Datasheet
Reviews (4)Q&A (2)References (47)

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Western blot - Anti-Calsequestrin antibody (ab3516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)

Key features and details

  • Rabbit polyclonal to Calsequestrin
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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概述

  • 产品名称

    Anti-Calsequestrin抗体
  • 描述

    兔多克隆抗体to Calsequestrin
  • 宿主

    Rabbit
  • 特异性

    This antibody recognizes both cardiac and skeletal muscle calsequestrin.
  • 经测试应用

    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Other Immunogen Type corresponding to Dog Calsequestrin. The immunogen is purified canine cardiac calsequestrin.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

Our Abpromise guarantee covers the use of ab3516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use at an assay dependent concentration.
WB 1/1000 - 1/10000.

This antibody detects an ~55 kDa protein representing Calsequestrin from canine cardiac extract. Additional bands at 97 kDa may be observed and have been reported to be Calsequestrin-like proteins.

IHC-P 1/100 - 1/500.

靶标

  • 功能

    Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. Also binds laminin.
  • 序列相似性

    Belongs to the calsequestrin family.
  • 细胞定位

    Sarcoplasmic reticulum lumen. Mitochondrion. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells. Also mitochondrial according to PubMed:7945294.
  • Target information above from: UniProt accession P31415 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 844 Human
    • Entrez Gene: 845 Human
    • Entrez Gene: 12372 Mouse
    • Entrez Gene: 12373 Mouse
    • Entrez Gene: 29209 Rat
    • Omim: 114250 Human
    • Omim: 114251 Human
    • SwissProt: O14958 Human
    • SwissProt: P31415 Human
    • SwissProt: O09161 Mouse
    • SwissProt: O09165 Mouse
    • SwissProt: P19633 Rat
    • SwissProt: P51868 Rat
    • Unigene: 57975 Human
    • Unigene: 632476 Human
    • Unigene: 12829 Mouse
    see all
  • 别名

    • Calmitin antibody
    • Calsequestrin 1 antibody
    • Calsequestrin 1 fast twitch skeletal muscle antibody
    • Calsequestrin 2 antibody
    • Calsequestrin 2 fast twitch cardiac muscle antibody
    • Calsequestrin antibody
    • Calsequestrin cardiac muscle isoform antibody
    • Calsequestrin skeletal muscle isoform antibody
    • Calsequestrin-1 antibody
    • Cardiac calsequestrin 2 antibody
    • CASQ 1 antibody
    • CASQ 2 antibody
    • Casq1 antibody
    • CASQ1_HUMAN antibody
    • PDIB1 antibody
    • PDIB2 antibody
    • Skeletal muscle calsequestrin 1 antibody
    • skeletal muscle isoform antibody
    see all

图片

  • Western blot - Anti-Calsequestrin antibody (ab3516)
    Western blot - Anti-Calsequestrin antibody (ab3516)
    All lanes : Anti-Calsequestrin antibody (ab3516) at 1/5000 dilution

    Lane 1 : RD (Human muscle rhabdomyosarcoma cell line) whole cell lysate
    Lane 2 : L6 (Rat skeletal muscle cell line) whole cell lysate
    Lane 3 : Mouse heart tissue lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : HRP-conjugated secondary antibody

    Observed band size: 55 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)

    ab3516 labelling Calsequestrin in the cytoplasm of human skeletal muscle tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)
    Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)

    Immunofluorescent analysis of Calsequestrin (green) showing staining in the cytoplasm and nucleus of C2C12 (Mouse myoblast cell line) cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab3516 in 3% BSA-PBS at a dilution of 1/100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS at room temperature in the dark. F-actin (red) was stained with a fluorescent red phalloidin and nuclei (blue) were stained with Hoechst or DAPI. Images were taken at a magnification of 60x.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)

    ab3516 labelling Calsequestrin in the cytoplasm of mouse heart tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)

    ab3516 labelling Calsequestrin in the cytoplasm of human heart tissue (right) compared with a negative control (left) by Immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS. Tissue sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin antibody (ab3516)

    ab3516 (1µg/ml) staining Calsequestrin in human skeletal muscle using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of discrete organelles within the cytoplasm.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)
    Immunocytochemistry/ Immunofluorescence - Anti-Calsequestrin antibody (ab3516)This image is courtesy of an anonymous Abreview

    ab3516 staining Calsequestrin in mouse myocyte cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 10% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/50 in serum) for 18 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
  • 文献 (47)

    发表研究结果有使用 ab3516?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3516 被引用在 47 文献中.

    • Eckhardt J  et al. Molecular basis of impaired extraocular muscle function in a mouse model of congenital myopathy due to compound heterozygous Ryr1 mutations. Hum Mol Genet 29:1330-1339 (2020). PubMed: 32242214
    • Asghari P  et al. Cardiac ryanodine receptor distribution is dynamic and changed by auxiliary proteins and post-translational modification. Elife 9:N/A (2020). PubMed: 31916935
    • Michailowsky V  et al. Defects in sarcolemma repair and skeletal muscle function after injury in a mouse model of Niemann-Pick type A/B disease. Skelet Muscle 9:1 (2019). PubMed: 30611303
    • Christiansen D  et al. A fast, reliable and sample-sparing method to identify fibre types of single muscle fibres. Sci Rep 9:6473 (2019). PubMed: 31019216
    • Wang Q  et al. Two pools of IRE1a in cardiac and skeletal muscle cells. FASEB J N/A:fj201802626R (2019). PubMed: 31051095
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-6 of 6 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Sample
    Mouse Cell (myocyte)
    Specification
    myocyte
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 14 2013

    Western blot

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Reduced Denaturing (8)
    Sample
    Human Tissue lysate - whole (Heart)
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 13 2013

    Western blot abreview for Anti-Calsequestrin antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Tissue lysate - whole (heart)
    Loading amount
    7.5 µg
    Specification
    heart
    Gel Running Conditions
    Reduced Denaturing (7.5%)
    Blocking step
    Invitrogen 00-0105 membrane blocking agent as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Apr 05 2013

    Question

    Can you tell me which abcam product best fits the inquiry attached?  I think it is ab3516 I also found ab97474 and ab32801.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 04 2011

    Answer

    I would recommened ab3516, it has been tested in more applications and species.

    Read More

    Abcam Scientific Support

    回复于 Nov 04 2011

    Western blot abreview for Anti-Calsequestrin antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (heart, ventricular tissue)
    Loading amount
    60 µg
    Specification
    heart, ventricular tissue
    Gel Running Conditions
    Reduced Denaturing (4~12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 02 2008

    Question

    Wondering if you could inform us about the target sequence for the rabbit polyclonal to Calsequestrin (ab3516)antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 25 2007

    Answer

    Thank you for your enquiry. This anti-Calsequestrin antibody (ab3516) was created against purified canine cardiac calsequestrin. Unfortunately, the exact epitope sequence has not been mapped at this time. I am sorry I can't be of more assistance in this occasion but should you require any further information, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Jun 25 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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