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Tags & Cell Markers Subcellular Markers Organelles ER

Anti-Calreticulin抗体- ER Marker (ab2907)

  • Datasheet
  • SDS
Reviews (16)Q&A (3)References (163)

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Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

Key features and details

  • Rabbit polyclonal to Calreticulin - ER Marker
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-Calreticulin antibody [EPR3924] - ER Marker (ab92516)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Calreticulin抗体- ER Marker
    参阅全部 Calreticulin 一抗
  • 描述

    兔多克隆抗体to Calreticulin - ER Marker
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Calreticulin.

  • 阳性对照

    • WB: HL-60, LNCaP, HeLa and MCF-7 cell lysates; Mouse and rat liver tissue lysates; Mouse skeletal muscle whole cell lysate. ICC/IF: A431, HeLa, U2OS, HepG2 and HMVEC cells.
  • 常规说明

      

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • ER
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Rabbit Calreticulin protein (ab15729)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Rabbit Calreticulin protein (ab15729)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2907于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF (8)
Use at an assay dependent concentration.
WB (4)
1/1000.
说明
ICC/IF
Use at an assay dependent concentration.
WB
1/1000.

靶标

  • 功能

    Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export.
  • 序列相似性

    Belongs to the calreticulin family.
  • 结构域

    Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
    The interaction with glycans occurs through a binding site in the globular lectin domain.
    The zinc binding sites are localized to the N-domain.
    Associates with PDIA3 through the tip of the extended arm formed by the P-domain.
  • 细胞定位

    Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes.
  • Target information above from: UniProt accession P27797 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 811 Human
    • Entrez Gene: 12317 Mouse
    • Entrez Gene: 64202 Rat
    • Omim: 109091 Human
    • SwissProt: P27797 Human
    • SwissProt: P14211 Mouse
    • SwissProt: P18418 Rat
    • Unigene: 515162 Human
    • Unigene: 1971 Mouse
    • Unigene: 467043 Mouse
    • Unigene: 974 Rat
    see all
  • 别名

    • Autoantigen RO antibody
    • CALR antibody
    • CALR protein antibody
    • CALR_HUMAN antibody
    • Calregulin antibody
    • Calreticulin antibody
    • cC1qR antibody
    • CRP55 antibody
    • CRT antibody
    • CRTC antibody
    • Endoplasmic reticulum resident protein 60 antibody
    • Epididymis secretory sperm binding protein Li 99n antibody
    • ERp60 antibody
    • FLJ26680 antibody
    • grp60 antibody
    • HACBP antibody
    • HEL S 99n antibody
    • RO antibody
    • Sicca syndrome antigen A (autoantigen Ro; calreticulin) antibody
    • Sicca syndrome antigen A antibody
    • SSA antibody
    see all

图片

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : LNCaP cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : MCF-7 cell lysate
    Lane 5 : Mouse liver tissue lysate
    Lane 6 : Rat liver tissue lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Observed band size: 55 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 3. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.
    ab2907 used at a 1/1000 dilution staining Calreticulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    HeLa cells were transfected overnight with empty vector or plasmids encoding the indicated IFITM3 constructs. Immunofluorescence with a-HA antibodies allowed IFITM3 visualization, and a-calreticulin staining allowed visualization of the ER. TOPRO-3 was used to visualize nuclei. Scale bars indicate 10 µm. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine.
  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)
    Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
    All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution

    All lanes : Whole cell lysate prepared from mouse skeletal muscle

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated mouse polyclonal to rabbit Ig at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 3 seconds

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (1:300) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemsitry/Immunofluorescence analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X for 30 minutes at room temperature. Cells were incubated with ab2907 (1:50) for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (1:300) in PBS and incubated for 30 minutes. Nuclei (blue) were stained with Hoechst 33342 dye (1µg/mL). Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (1:120) and nuclei (red) were stained with DRAQ5 (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)

    Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labelling Calreticulin using ab2907.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)
    Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous Abreview
    Immunofluorescence analysis of HepG2 cells, staining Calreticulin with ab2907.

    Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% saponin) for 1 hour at 20°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

实验方案

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (163)

发表研究结果有使用 ab2907?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2907 被引用在 163 文献中.

  • Fu D  et al. T cell recruitment triggered by optimal dose platinum compounds contributes to the therapeutic efficacy of sequential PD-1 blockade in a mouse model of colon cancer. Am J Cancer Res 10:473-490 (2020). PubMed: 32195021
  • Yang J  et al. Dinaciclib prolongs survival in the LSL-KrasG12D/+ ; LSL-Trp53R172H/+ ; Pdx-1-Cre (KPC) transgenic murine models of pancreatic ductal adenocarcinoma. Am J Transl Res 12:1031-1043 (2020). PubMed: 32269732
  • Scherr AL  et al. Knockdown of Atg7 Induces Nuclear-LC3 Dependent Apoptosis and Augments Chemotherapy in Colorectal Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32046105
  • Song S  et al. Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia. Cell Death Dis 11:109 (2020). PubMed: 32034134
  • Pacheco A  et al. Mechanism and role of the intra-axonal Calreticulin translation in response to axonal injury. Exp Neurol 323:113072 (2020). PubMed: 31669485
View all Publications for this product

客户评价及客户问答

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-Calreticulin antibody - ER Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Hela cells)
Gel Running Conditions
Non-reduced Denaturing (premade 4-12%)
Loading amount
15 µg
Specification
Hela cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Read More

Mrs. Federica Ferrentino

Verified customer

提交于 Mar 05 2020

Western blot abreview for Anti-Calreticulin antibody - ER Marker

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Arabidopsis thaliana Cell lysate - whole cell (cell culture)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
cell culture
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Joshua Heazlewood

Verified customer

提交于 Jul 14 2011

Western blot abreview for Anti-Calreticulin antibody - ER Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (skeletal muscle)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
skeletal muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Read More

Abcam user community

Verified customer

提交于 Oct 17 2008

Western blot abreview for Anti-Calreticulin antibody - ER Marker

Good
Abreviews
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (ER fraction)
Gel Running Conditions
Reduced Denaturing (gradient 4-20%)
Loading amount
10 µg
Specification
ER fraction
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Aug 22 2008

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