Anti-Calreticulin抗体(ab94935)
Key features and details
- Chicken polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calreticulin抗体
参阅全部 Calreticulin 一抗 -
描述
鸡多克隆抗体to Calreticulin -
宿主
Chicken -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rabbit, Cow, Pig, Zebrafish, Cynomolgus monkey -
免疫原
Synthetic peptide corresponding to Mouse Calreticulin aa 24-43.
Sequence:KEQFLDGDAWTNRWVESKHK
(Peptide available asab5838) -
阳性对照
- WB: A-549, HEK-293 cell lysate; Mouse Brain lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94935于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 60-66 kDa (predicted molecular weight: 48 kDa). |
靶标
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功能
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
序列相似性
Belongs to the calreticulin family. -
结构域
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
细胞定位
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 281036 Cow
- Entrez Gene: 102121336 Cynomolgus monkey
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 100381266 Pig
- Entrez Gene: 100009050 Rabbit
- Entrez Gene: 30248 Zebrafish
- Omim: 109091 Human
see all -
别名
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
图片
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All lanes : Anti-Calreticulin antibody (ab94935) at 1/2000 dilution
Lane 1 : A-549 cell lysate
Lane 2 : HEK-293 cell lysate
Lane 3 : Mouse Brain lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/ml
Predicted band size: 48 kDa
数据表及文件
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Datasheet download
文献 (1)
ab94935 被引用在 1 文献中.
- Stamm CE et al. Screening Mycobacterium tuberculosis Secreted Proteins Identifies Mpt64 as a Eukaryotic Membrane-Binding Bacterial Effector. mSphere 4:N/A (2019). PubMed: 31167949