概述

  • 产品名称
    Anti-Calpain 2抗体[1E8]
    参阅全部 Calpain 2 一抗
  • 描述
    小鼠单克隆抗体[1E8] to Calpain 2
  • 宿主
    Mouse
  • 特异性
    Recognises a linear epitope of the 80 kDa (large) subunit of bovine m-calpain
  • 经测试应用
    适用于: ICC/IF, ELISA, WBmore details
  • 种属反应性
    与反应: Cow, Human
  • 免疫原

    Bovine cardiac Calpain 2.

性能

应用

Our Abpromise guarantee covers the use of ab713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 0.5 µg/ml. Fix with 2% paraformaldehyde.
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 µg/ml.

靶标

  • 功能
    Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.
  • 组织特异性
    Ubiquitous.
  • 序列相似性
    Belongs to the peptidase C2 family.
    Contains 1 calpain catalytic domain.
    Contains 3 EF-hand domains.
  • 细胞定位
    Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Calcium activated neutral proteinase 2 antibody
    • Calcium activated neutral proteinase antibody
    • Calcium-activated neutral protease 2, catalytic subunit antibody
    • Calcium-activated neutral proteinase 2 antibody
    • CALP80 antibody
    • Calpain 2 (m/II) large subunit antibody
    • Calpain 2 catalytic subunit antibody
    • Calpain 2 large catalytic subunit antibody
    • Calpain 2 large subunit antibody
    • Calpain 2, large [catalytic] subunit antibody
    • Calpain large polypeptide L2 antibody
    • Calpain M type antibody
    • Calpain M-type antibody
    • Calpain-2 catalytic subunit antibody
    • Calpain-2 large subunit antibody
    • Calpain2 antibody
    • CAN2_HUMAN antibody
    • CANP 2 antibody
    • CANP L2 antibody
    • CANP2 antibody
    • CANPL 2 antibody
    • CANPL2 antibody
    • CANPml antibody
    • Capa2 antibody
    • CAPN 2 antibody
    • CAPN2 antibody
    • FLJ39928 antibody
    • M calpain antibody
    • M calpin antibody
    • M type antibody
    • M-calpain antibody
    • mCANP antibody
    • Millimolar calpain antibody
    • Millimolar-calpain antibody
    see all

图片

  • Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.

    Immunodetection of m-calpain using MAb 1E8 (1) in Western blot.
  • Immunoflurescence of intracellular calpain in bovine lens epithel cells using MAb 1E8 (green).

文献

ab713 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Answer

I am sorry that the vial you received was contaminated and can confirm that a replacement vial will be sent to you this afternoon on order 241265. If you still experience problems with the new vial please provide your protocol details via the links below and we will look into this matter and see if we can provide suggestions to improve the results. If the problem is antibody related we will be happy to offer a refund on your original order if you contact us within 120 days of receipt of the new vial and if the antibody doesn't perform as described on the datasheet. For IHC: https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=713&mode=questionaire For WB: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=713&mode=questionaire I would like to also encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. We reward each Abreview with Abpoints which can be used for discounts off future purchases and gifts. To find out more about our Abreview system, please see the following URL: https://www.abcam.com/abreviews

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Cow Cell (chondrocytes and fibrochondrocytes)
Specification
chondrocytes and fibrochondrocytes
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2%

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Verified customer

提交于 Mar 09 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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