Anti-Calpain 1抗体(ab28258)
Key features and details
- Rabbit polyclonal to Calpain 1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Calpain 1抗体
参阅全部 Calpain 1 一抗 -
描述
兔多克隆抗体to Calpain 1 -
宿主
Rabbit -
特异性
ab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.). -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human Calpain 1.
(Peptide available asab41299) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
存储溶液
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
The antibody has been peptide-affinity purified. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab28258于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.
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说明 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. |
靶标
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功能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
细胞定位
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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别名
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
图片
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Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution + Calpain 1 at 10 µg
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (24)
ab28258 被引用在 24 文献中.
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Sukocheva OA et al. Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres. Int J Mol Sci 22:N/A (2021). PubMed: 33919234
- Gritsyna YV et al. Inhibition of Histone Deacetylases 4 and 5 Reduces Titin Proteolysis and Prevents Reduction of TTN Gene Expression in Atrophied Rat Soleus Muscle after Seven-Day Hindlimb Unloading. Dokl Biochem Biophys 495:338-341 (2020). PubMed: 33368047
- Duquette PM & Lamarche-Vane N The calcium-activated protease calpain regulates netrin-1 receptor deleted in colorectal cancer-induced axon outgrowth in cortical neurons. J Neurochem 152:315-332 (2020). PubMed: 31344270
- Popova S et al. Predominant synthesis of giant myofibrillar proteins in striated muscles of the long-tailed ground squirrel Urocitellus undulatus during interbout arousal. Sci Rep 10:15185 (2020). PubMed: 32938992