Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (A20 B cells)
Permeabilization
Yes - 0.1% Triton
Specification
A20 B cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% FBS in PBS
Dilution
1/100
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 568 Goat anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Marcella Flores
Verified customer
提交于 Jan 10 2013