重组Anti-Calnexin抗体[EPR21205] (ab213243)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21205] to Calnexin
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Human
概述
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产品名称
Anti-Calnexin抗体[EPR21205]
参阅全部 Calnexin 一抗 -
描述
兔单克隆抗体[EPR21205] to Calnexin -
宿主
Rabbit -
经测试应用
适用于: WBmore details
不适用于: ICC/IF -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant full length protein (His-tag) corresponding to Mouse Calnexin. Expressed in HEK293 Cells
Database link: NP_031623.1 -
阳性对照
- WB: NIH3T3, HeLa, HAP1
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常规说明
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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克隆
单克隆 -
克隆编号
EPR21205 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) (ab150078)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
- Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
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Matched antibody pairs
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab213243于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa.
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说明 |
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WB
Use a concentration of 1 - 2.5 µg/ml. Predicted molecular weight: 68 kDa. |
靶标
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功能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
序列相似性
Belongs to the calreticulin family. -
细胞定位
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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别名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
图片
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Lane 1: NIH3T3 whole cell lysate (10 µg)
Lane 2: Mouse brain whole tissue lysate (10 µg)
Lane 3: HeLa whole cell lysate (10 µg)
Lane 4: Hap1 Wild-type whole cell lysate (10 µg)
Lane 5: CANX knockout Hap1 whole cell lysate (10 µg)Lanes 1 - 5: Merged signal (red and green). Green - ab213243 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab213243 was shown to specifically react with Calnexin in wild-type HAP1 cells as signal was lost in Calnexin (CANX) knockout cells. Wild-type and CANX knockout samples were subjected to SDS-PAGE. Nitrocellulose membranes were blocked in 3% milk in TBST before ab213243 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1µg/mL and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab213243 被引用在 6 文献中.
- Pinson MR et al. Gag-like proteins: Novel mediators of prenatal alcohol exposure in neural development. Alcohol Clin Exp Res 46:556-569 (2022). PubMed: 35187673
- Kumar P et al. Tumour-associated antigenic peptides are present in the HLA class I ligandome of cancer cell line derived extracellular vesicles. Immunology 166:249-264 (2022). PubMed: 35318648
- Liu Z et al. Exosome-mediated transduction of mechanical force regulates prostate cancer migration via microRNA. Biochem Biophys Rep 31:101299 (2022). PubMed: 35812347
- Wang X et al. Exosome-delivered circRNA promotes glycolysis to induce chemoresistance through the miR-122-PKM2 axis in colorectal cancer. Mol Oncol 14:539-555 (2020). PubMed: 31901148
- Xue J et al. Addition of High Molecular Weight Hyaluronic Acid to Fibroblast-Like Stromal Cells Modulates Endogenous Hyaluronic Acid Metabolism and Enhances Proteolytic Processing and Secretion of Versican. Cells 9:N/A (2020). PubMed: 32668663
- Fan SJ et al. Glutamine deprivation alters the origin and function of cancer cell exosomes. EMBO J 39:e103009 (2020). PubMed: 32720716