Anti-Calcium Pump PMCA2 ATPase抗体(ab3529)
Key features and details
- Rabbit polyclonal to Calcium Pump PMCA2 ATPase
- Suitable for: IHC-P
- Reacts with: Rat, Chicken, Human, Pig, Non human primates
- Isotype: IgG
概述
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产品名称
Anti-Calcium Pump PMCA2 ATPase抗体 -
描述
兔多克隆抗体to Calcium Pump PMCA2 ATPase -
宿主
Rabbit -
特异性
Detects PMCA 2a and 2b ATPase. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Rat, Chicken, Human, Pig, Non human primates -
免疫原
Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 5-19.
Sequence:TNSDFYSKNQRNESS
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. |
靶标
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功能
This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell. -
组织特异性
Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle. -
序列相似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 491 Human
- Entrez Gene: 24215 Rat
- Omim: 108733 Human
- SwissProt: Q01814 Human
- SwissProt: P11506 Rat
- Unigene: 268942 Human
- Unigene: 90982 Rat
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别名
- AT2B2_HUMAN antibody
- atp2b2 antibody
- ATPase plasma membrane Ca2+ transporting 2 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)This image is courtesy of an Abreview submitted by Pradeep Sherkhaneab3529 staining Calcium Pump PMCA2 ATPase in Mouse purkinje cells in cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS + 3% NGS + 0.5% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.
实验方案
数据表及文件
文献 (9)
ab3529 被引用在 9 文献中.
- Robertson SYT et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
- Schmidt N et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
- Sun F et al. 1,25-Dihydroxyvitamin D3 modulates calcium transport in goat mammary epithelial cells in a dose- and energy-dependent manner. J Anim Sci Biotechnol 7:41 (2016). WB ; Goat . PubMed: 27471592
- Murphy S et al. Label-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy. Clin Proteomics 12:27 (2015). PubMed: 26604869
- Laporta J et al. Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice. PLoS One 9:e110190 (2014). PubMed: 25299122
- Sherkhane P & Kapfhammer JP The Plasma Membrane Ca(2+)-ATPase2 (PMCA2) Is Involved in the Regulation of Purkinje Cell Dendritic Growth in Cerebellar Organotypic Slice Cultures. Neural Plast 2013:321685 (2013). IHC (PFA fixed) ; Mouse . PubMed: 24288624
- Chen Q et al. The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells. Eur J Neurosci 36:2302-10 (2012). IHC ; Rat . PubMed: 22672315
- Kurnellas MP et al. Reduced expression of plasma membrane calcium ATPase 2 and collapsin response mediator protein 1 promotes death of spinal cord neurons. Cell Death Differ : (2010). WB, ICC/IF ; Rat . PubMed: 20489728
- Jensen TP et al. Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3. J Physiol 579:85-99 (2007). WB ; Rat . PubMed: 17170045
