为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Signal Transduction Metabolism Plasma Membrane ATPases

Anti-Calcium Pump PMCA2 ATPase抗体(ab3529)

  • Datasheet
Reviews (3) Submit a question References (9)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)

Key features and details

  • Rabbit polyclonal to Calcium Pump PMCA2 ATPase
  • Suitable for: IHC-P
  • Reacts with: Human, Non human primates
  • Isotype: IgG

你可能会感兴趣

生物化学
Product image
Bafilomycin A1, (V)-ATPase inhibitor (ab120497)
二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-Calcium Pump PMCA2 ATPase抗体
  • 描述

    兔多克隆抗体to Calcium Pump PMCA2 ATPase
  • 宿主

    Rabbit
  • 特异性

    Detects PMCA 2a and 2b ATPase.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Calcium Pump PMCA2 ATPase aa 1-100.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Plasma Membrane
    • ATPases
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
IHC-P
Human
应用 Ab评论 说明
IHC-P (1)
Use at an assay dependent concentration.
说明
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
  • 组织特异性

    Mainly expressed in brain cortex. Found in low levels in skeletal muscle, heart muscle, stomach, liver, kidney and lung. Isoforms containing segment B are found in brain cortex and at low levels in other tissues. Isoforms containing segments X and W are found at low levels in all tissues. Isoforms containing segment A and segment Z are found at low levels in skeletal muscle and heart muscle.
  • 序列相似性

    Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily.
  • 细胞定位

    Cell membrane.
  • Target information above from: UniProt accession Q01814 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 491 Human
    • Omim: 108733 Human
    • SwissProt: Q01814 Human
    • Unigene: 268942 Human
    • 别名

      • AT2B2_HUMAN antibody
      • atp2b2 antibody
      • ATPase plasma membrane Ca2+ transporting 2 antibody
      • Plasma membrane calcium ATPase 2 antibody
      • Plasma membrane calcium ATPase antibody
      • Plasma membrane calcium ATPase isoform 2 antibody
      • Plasma membrane calcium pump isoform 2 antibody
      • Plasma membrane calcium transporting ATPase 2 antibody
      • Plasma membrane calcium-transporting ATPase 2 antibody
      • PMCA2 antibody
      • PMCA2a antibody
      • PMCA2i antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)This image is courtesy of an Abreview submitted by Pradeep Sherkhane

      ab3529 staining Calcium Pump PMCA2 ATPase in Mouse purkinje cells in cerebellum tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/500 in PBS + 3% NGS + 0.5% Triton X-100) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG monoclonal (1/500) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
      Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA2 ATPase antibody (ab3529)
      Immunohistochemistry was performed on cancer biopsies of deparaffinized Human cervical carcinoma tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a rabbit polyclonal antibody recognizing PMCA2 ATPase ab3529 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    实验方案

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (9)

    发表研究结果有使用 ab3529?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3529 被引用在 9 文献中.

    • Robertson SYT  et al. Multiple Calcium Export Exchangers and Pumps Are a Prominent Feature of Enamel Organ Cells. Front Physiol 8:336 (2017). WB . PubMed: 28588505
    • Schmidt N  et al. Neuroplastin and Basigin Are Essential Auxiliary Subunits of Plasma Membrane Ca2+-ATPases and Key Regulators of Ca2+ Clearance. Neuron 96:827-838.e9 (2017). PubMed: 29056295
    • Sun F  et al. 1,25-Dihydroxyvitamin D3 modulates calcium transport in goat mammary epithelial cells in a dose- and energy-dependent manner. J Anim Sci Biotechnol 7:41 (2016). WB ; Goat . PubMed: 27471592
    • Murphy S  et al. Label-free mass spectrometric analysis reveals complex changes in the brain proteome from the mdx-4cv mouse model of Duchenne muscular dystrophy. Clin Proteomics 12:27 (2015). PubMed: 26604869
    • Laporta J  et al. Peripheral serotonin regulates maternal calcium trafficking in mammary epithelial cells during lactation in mice. PLoS One 9:e110190 (2014). PubMed: 25299122
    • Sherkhane P & Kapfhammer JP The Plasma Membrane Ca(2+)-ATPase2 (PMCA2) Is Involved in the Regulation of Purkinje Cell Dendritic Growth in Cerebellar Organotypic Slice Cultures. Neural Plast 2013:321685 (2013). IHC (PFA fixed) ; Mouse . PubMed: 24288624
    • Chen Q  et al. The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells. Eur J Neurosci 36:2302-10 (2012). IHC ; Rat . PubMed: 22672315
    • Kurnellas MP  et al. Reduced expression of plasma membrane calcium ATPase 2 and collapsin response mediator protein 1 promotes death of spinal cord neurons. Cell Death Differ : (2010). WB, ICC/IF ; Rat . PubMed: 20489728
    • Jensen TP  et al. Presynaptic plasma membrane Ca2+ ATPase isoform 2a regulates excitatory synaptic transmission in rat hippocampal CA3. J Physiol 579:85-99 (2007). WB ; Rat . PubMed: 17170045

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calcium Pump PMCA2 ATPase antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Antigen retrieval step
    None
    Sample
    Mouse Tissue sections (Purkinje cells in cerebellum)
    Specification
    Purkinje cells in cerebellum
    Permeabilization
    Yes - 0.5% Triton x100
    Fixative
    Paraformaldehyde
    Read More

    Dr. Pradeep Sherkhane

    Verified customer

    提交于 Jul 08 2013

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calcium Pump PMCA2 ATPase antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue lysate - whole (Rat cerebellar slices)
    Specification
    Rat cerebellar slices
    Fixative
    Formaldehyde
    Antigen retrieval step
    None
    Blocking step
    Serum as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1%
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 17 2005

    Western blot abreview for Anti-Calcium Pump PMCA2 ATPase antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Rat Tissue lysate - other (Rat forebrain and cerebellum preparations, eg syna)
    Specification
    Rat forebrain and cerebellum preparations, eg syna
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 17 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有