重组Anti-Calcitonin抗体[SP17] (ab16697)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP17] to Calcitonin
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Calcitonin抗体[SP17]
参阅全部 Calcitonin 一抗 -
描述
兔单克隆抗体[SP17] to Calcitonin -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is considered to be commercially sensitive.
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阳性对照
- IHC-P: Human thyroid medullary carcinoma, pituitary and lung adenocarcinoma tissue. ICC/IF: TT cells
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常规说明
This product has switched from a hybridoma to recombinant production format on 22nd June 2020.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
SP17 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab16697于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/100.
|
|
ICC/IF |
1/50.
|
说明 |
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IHC-P
1/100. |
ICC/IF
1/50. |
靶标
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相关性
Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity. -
细胞定位
Cytoplasmic and Secreted -
数据库链接
- Entrez Gene: 796 Human
- Entrez Gene: 12310 Mouse
- Entrez Gene: 24241 Rat
- Omim: 114130 Human
- SwissProt: P01258 Human
- SwissProt: P70160 Mouse
- SwissProt: P01257 Rat
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别名
- CALC 1 antibody
- CALC A antibody
- CALC1 antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Calcitonin with purified ab16697 at 1/50 (2.4 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human thyroid medullary carcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human pituitary tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
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Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.
This image was generated using the hybridoma version of the product.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab16697 被引用在 1 文献中.
- Takeshima K et al. False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization. Endocr J 67:1007-1017 (2020). PubMed: 32522910