Anti-CAD抗体(ab99313)
Key features and details
- Rabbit polyclonal to CAD
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-CAD抗体
参阅全部 CAD 一抗 -
描述
兔多克隆抗体to CAD -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, IPmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human CAD aa 1850-2000.
Database link: NP_004332.2 -
阳性对照
- WB: K-562, Jurkat, HeLa, 293T and NIH3T3 whole cell lysates. IP: Jurkat cell lysate. IHC-P: Human ovarian carcinoma.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab99313 was affinity purified using an epitope specific to CAD immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab99313于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/200.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 243 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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说明 |
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IHC-P
1/200. |
WB
1/2000 - 1/10000. Predicted molecular weight: 243 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
靶标
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相关性
Carbamoyl phosphate synthetase-aspartate carbamoyltransferase-dihydroorotase (CAD) is a multifunctional protein that initiates and regulates mammalian de novo pyrimidine biosynthesis. This trifunctional protein which is associated with the enzymatic activities of the first 3 enzymes in the 6-step pathway of pyrimidine biosynthesis is the rate-limiting step in the de novo pyrimidine synthetic pathway. Although most of the CAD protein in the cell is cytosolic, phosphorylation at threonine 456 localizes the protein to the nucleus. While MAPK and EGF phosphorylate CAD at threonine 456, MAPK and c-myc have been found to induce over-expression of CAD. -
细胞定位
Cytoplasmic and Nuclear -
数据库链接
- Entrez Gene: 790 Human
- Entrez Gene: 69719 Mouse
- Omim: 114010 Human
- SwissProt: P27708 Human
- SwissProt: B2RQC6 Mouse
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别名
- Aspartate carbamoyltransferase antibody
- Aspartate transcarbamylase antibody
- CAD protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling CAD with ab99313 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-CAD antibody (ab99313) at 0.04 µg/ml
Lane 1 : HEK-293T whole cell lysate
Lane 2 : K562 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH/3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 243 kDa
Exposure time: 10 seconds -
CAD Antibody | Bethyl Laboratories, Inc.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab99313 尚未被引用在任何文献中。