常规说明Cell line: Caco-2 (Human colorectal adenocarcinoma).
Growth media: Minimum essential medium (Eagle) with 2mM L-glutamine, 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, 1.0 mM sodium pyruvate, and 20% FBS.
经测试应用适用于: WBmore details
存放说明Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
存储溶液Constituent: 5% Beta mercaptoethanol
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背景Caco-2 cells possess many of the properties of the small intestine. As such, these cells represent a useful and well-accepted tool for metabolism and transport studies (absorption, permeability and/or secretion of drugs/chemicals across the intestinal mucosa).
Our Abpromise guarantee covers the use of ab3950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
Caco-2 cell lysate is ready to load on SDS-PAGE for Western blotting, 10 - 20 µg per lane isr ecommended for mini gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.