Anti-CACNA1S抗体[1A] (ab2862)

Reviews (2)Q&A (5)References (16)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)
  • Flow Cytometry - Anti-CACNA1S antibody [1A] (ab2862)

Key features and details

  • Mouse monoclonal [1A] to CACNA1S
  • Suitable for: WB, ELISA, Inhibition Assay, ICC/IF, Flow Cyt, Flow Cyt (Intra), IP, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Rabbit, Guinea pig, Human
  • Isotype: IgG1

概述

  • 产品名称

    Anti-CACNA1S抗体[1A]
    参阅全部 CACNA1S 一抗

  • 描述

    小鼠单克隆抗体[1A] to CACNA1S

  • 宿主

    Mouse

  • 经测试应用

    适用于: WB, ELISA, Inhibition Assay, ICC/IF, Flow Cyt, Flow Cyt (Intra), IP, IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Mouse, Rat, Rabbit, Guinea pig, Human

  • 免疫原

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • Human skeletal muscle tissue

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    pH: 7.4
    Preservative: 0.05% Sodium azide
  • Concentration information loading...

  • 纯度

    Protein A purified

  • Primary antibody说明

    Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.

  • 克隆

    单克隆

  • 克隆编号

    1A

  • 同种型

    IgG1

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2862于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration. PubMed: 21693436
Flow Cyt
Use a concentration of 1 µg/ml.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
IHC-P
1/20.
IHC-Fr
Use at an assay dependent concentration. PubMed: 21474431
说明
WB
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Inhibition Assay
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 21693436
Flow Cyt
Use a concentration of 1 µg/ml.
Flow Cyt (Intra)
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IP
Use at an assay dependent concentration.
IHC-P
1/20.
IHC-Fr
Use at an assay dependent concentration. PubMed: 21474431

靶标

  • 功能

    Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.

  • 组织特异性

    Skeletal muscle specific.

  • 疾病相关

    Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1) [MIM:170400]; also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels.
    Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
    Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1) [MIM:188580]. A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease.

  • 序列相似性

    Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.

  • 结构域

    Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
    The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.

  • 翻译后修饰

    Phosphorylation by PKA activates the calcium channel.

  • 细胞定位

    Membrane.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • alpha-1 polypeptide antibody
    • CAC1S_HUMAN antibody
    • CACH1 antibody
    • Cach1b antibody
    • CACN1 antibody
    • CACNA1S antibody
    • CACNL1A3 antibody
    • Calcium channel antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody
    • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody
    • Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody
    • Calcium channel, voltage dependent, L type, alpha 1S subunit antibody
    • Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody
    • Cav1.1 antibody
    • CCHL1A3 antibody
    • Dihydropyridine receptor antibody
    • Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody
    • fmd antibody
    • HOKPP antibody
    • HypoPP antibody
    • isoform 3 antibody
    • L type antibody
    • Malignant hyperthermia susceptibility 5 antibody
    • mdg antibody
    • MHS5 antibody
    • ROB1 antibody
    • sj antibody
    • skeletal muscle antibody
    • TTPP1 antibody
    • Voltage gated calcium channel subunit alpha Cav1.1 antibody
    • Voltage-dependent L-type calcium channel subunit alpha-1S antibody
    • Voltage-gated calcium channel subunit alpha Cav1.1 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

  • Flow Cytometry - Anti-CACNA1S antibody [1A] (ab2862)
    Flow Cytometry - Anti-CACNA1S antibody [1A] (ab2862)

    Flow cytometry analysis of Dihydropyridine Receptor alpha-1 in U251 cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5 x106 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a Dihydropyridine Receptor alpha-1 monoclonal antibody at 1 µg/test for 40 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated secondary antibody and re-suspended in PBS for FACS analysis.

文献 (16)

发表研究结果有使用 ab2862？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2862 被引用在 16 文献中.

  • Pelletier L  et al. In vivo RyR1 reduction in muscle triggers a core-like myopathy. Acta Neuropathol Commun 8:192 (2020). PubMed: 33176865
  • Wakeham CM  et al. Expression and distribution of trophoblast glycoprotein in the mouse retina. J Comp Neurol 528:1660-1671 (2020). PubMed: 31891182
  • Garibaldi M  et al. 'Dusty core disease' (DuCD): expanding morphological spectrum of RYR1 recessive myopathies. Acta Neuropathol Commun 7:3 (2019). PubMed: 30611313
  • Jokela M  et al. An unusual ryanodine receptor 1 (RYR1) phenotype: Mild calf-predominant myopathy. Neurology 92:e1600-e1609 (2019). PubMed: 30842289
  • Sébastien M  et al. Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction. Skelet Muscle 8:30 (2018). PubMed: 30231928
View all Publications for this product

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1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-CACNA1S antibody [1A]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - other (Skeletal muscle microsomes)
Gel Running Conditions
Reduced Denaturing (4–15%)
Loading amount
10 µg
Specification
Skeletal muscle microsomes
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

提交于 Jun 15 2015

Immunocytochemistry/ Immunofluorescence abreview for Anti-CACNA1S antibody [1A]

 Excellent
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (C2C12 myotube)
Permeabilization
Yes - 0.1% triton/PBS
Specification
C2C12 myotube
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Formaldehyde
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Abcam user community

Verified customer

提交于 Jun 15 2015

Question

What dilution was tested in IP with these antibodies?

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Answer

Thank you for your enquiry. Regarding the two antibodies, ab2862 and ab2825, I was able to find the specific antibody concentrations for you. The concentration of ab2825 (PMCA ATPase) is 7.7 mg/ml and the concentration of ab2862 (Dihydropyridine Receptor Alpha-1) is 3.8 mg/ml. I would recommend a range for immunoprecipitation assays, 10-500 ug of sample to 0.5-5 ug of antibody. Based on resources I have available, I would recommend starting in the mid-range. I hope this information helps. Please let me know if I can be of further assistance.

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Question

I wonder if I can use this antibody for a Western Blot with mouse skeletal muscle TT membranes. Will I have problems because of the anti mouse secondary antibody? What protocol do you recomend me for Western and IP?

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Answer

Thank you for your enquiry. This antibody has been shown to work by Western blot; detecting a ~200 kDa protein corresponding to the DHP receptor in rat skeletal muscle extracts. You shouldn't have too much of a problem with your IP and western experiments given that IgG migrates at ~50KDa and therefore wont interfere with your targeting of DHPR alpha 1. I would recommend the use of low leaching protein A agarose and localising your blot for all products >100KDa by cutting your membrane with a scalpel. I thought that I would mention that DHPR alpha 1 was detected in skeletal muscle in the following publication. http://www.jbc.org/cgi/reprint/277/52/50535 Zheng Z, Wang ZM, Delbono O. Insulin-like growth factor-1 increases skeletal muscle dihydropyridine receptor alpha 1S transcriptional activity by acting on the cAMP-response element-binding protein element of the promoter region. J Biol Chem. 2002 Dec 27;277(52):50535-42. Epub 2002 Oct 28. Erratum in: J Biol Chem. 2003 May 2;278(18):16453. PMID: 12407098 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

Anonymous Abreview submitted on 28 November 2005: DHPR alpha 1 antibody [1A] (ab2862): Sample: Mouse Tissue sections (skeleton muscles) Application: Immunohistochemistry (Frozen sections) Blocking step:: BSA as blocking agent for 1 hour(s)  0 minute(s)  Concentration: 2% Fixation:: Paraformaldehyde Antigen retrieval step:: None Incubation time:: 20 hour(s)  0 minute(s)  Positive control:: none Negative control:: omit the primer antibody. Dilution: 1/200 Rating: Inconclusive Additional notes: It is pity. My tissue sample is from mouse. We are trying to conjugate it. Secondary antibody: Name:: Non-Abcam one was used: Fluorescein (FITC) AffiniPure F(ab')2 Fragment Goa Conjugation: FITC Dilution:: 1/100

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Answer

Thank you for your enquiry. I am sorry that ab2862 is not working for you. Since you are using the antibody in a tested application for a species that the antibody has been shown to cross-react with, this should work. Without knowing the exact problem you are having, I can only make general suggestions assuming you are seeing no staining. Have you tried blocking with 10% goat serum in place of BSA? You may wish to try this for 30 minutes at room temperature. In addition, you may wish to try incubating with the primary antibody overnight at 4 degrees. If you could give me further details of the protocol you are using by submitting the form below, I would be happy to look into this further and try to help you to get this antibody to work. https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2862&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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Question

To whom it may concern, In order to make immunoprecipitation experiments with the mAb 1a against L-type calcium channel (DHPR) alpha1s subunit (Catalog ab2862) I need to know the concentration and or the Molecular weight of the Ab. Could you please try to give me some information about that. Sincerely

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Answer

Thank you for your enquiry. The concentration of ab2862 is 4.4 mg/ml.

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Question

Customer would like to know if ab2862 is n- or c-terminal, and if there are any details about it was tested in IHC.

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Answer

This antibody has not been epitope mapped. The following are references in regards to IHC: Neuron, 5: 339-351, 1990. J. Cell Biol., 123(5): 1161-1174, 1993. Am. J. Physiol., 268(37): F251-F257, 1995. If you have any more questions, please contact us again.

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