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Neuroscience Neurotransmission Calcium Signaling Calcium Channels L-type

Anti-CACNA1S抗体[1A] (ab2862)

  • Datasheet
Reviews (2)Q&A (5)References (12)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)

    Key features and details

    • Mouse monoclonal [1A] to CACNA1S
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    概述

    • 产品名称

      Anti-CACNA1S抗体[1A]
      参阅全部 CACNA1S 一抗
    • 描述

      小鼠单克隆抗体[1A] to CACNA1S
    • 宿主

      Mouse
    • 经测试应用

      适用于: IHC-Pmore details
    • 种属反应性

      与反应: Human
    • 免疫原

      Full length native protein (purified) corresponding to Rabbit CACNA1S. Purified from rabbit muscle T-tubule dihydropyridine receptor.

    • 阳性对照

      • Human skeletal muscle tissue
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      pH: 7.4
      Preservative: 0.05% Sodium azide
    • Concentration information loading...
    • 纯度

      Protein A purified
    • Primary antibody说明

      Voltage-sensitive calcium channels mediate the entry of calcium into many types of excitable cells and thus play a key role in neurotransmitter release and excitation-contraction (E-C) coupling. The 1,4-dihydropyridines (DHPs) are synthetic organic compounds which can be used to identify the L-type calcium channels that are found in all types of vertebrate muscle, neuronal and neuroendocrine cells. The DHP receptor is part of the L-type calcium channel complex and is thought to be the voltage sensor in E-C coupling. The purified DHP receptor isolated from triads is composed of at least four subunits. The alpha-1 subunit contains the binding site for the DHPs and shows high sequence homology to the voltage gated sodium channel. The alpha-2 subunit is a large glycoprotein associated with the DHP receptor which was first described in skeletal muscle and is also found in high concentrations in other excitable tissues such as cardiac muscle and brain and in low concentrations in most other tissues studied. The other two subunits that co-purify with the DHP receptor are termed beta and gamma.
    • 克隆

      单克隆
    • 克隆编号

      1A
    • 同种型

      IgG1
    • 研究领域

      • Neuroscience
      • Neurotransmission
      • Calcium Signaling
      • Calcium Channels
      • L-type
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calcium Channels
      • Cardiovascular
      • Heart
      • Cardiac arrhythmias

    相关产品

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

    应用

    Our Abpromise guarantee covers the use of ab2862 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    IHC-P 1/20.

    靶标

    • 功能

      Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1S gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1S subunit play an important role in excitation-contraction coupling in skeletal muscle.
    • 组织特异性

      Skeletal muscle specific.
    • 疾病相关

      Defects in CACNA1S are the cause of periodic paralysis hypokalemic type 1 (HOKPP1) [MIM:170400]; also designated HYPOPP. HOKPP1 is an autosomal dominant disorder manifested by episodic flaccid generalized muscle weakness associated with falls of serum potassium levels.
      Defects in CACNA1S are the cause of malignant hyperthermia susceptibility type 5 (MHS5) [MIM:601887]; an autosomal dominant disorder that is potentially lethal in susceptible individuals on exposure to commonly used inhalational anesthetics and depolarizing muscle relaxants.
      Defects in CACNA1S are the cause of susceptibility to thyrotoxic periodic paralysis type 1 (TTPP1) [MIM:188580]. A sporadic muscular disorder characterized by episodic weakness and hypokalemia during a thyrotoxic state. It is clinically similar to hereditary hypokalemic periodic paralysis, except for the fact that hyperthyroidism is an absolute requirement for disease manifestation. The disease presents with recurrent episodes of acute muscular weakness of the four extremities that vary in severity from paresis to complete paralysis. Attacks are triggered by ingestion of a high carbohydrate load or strenuous physical activity followed by a period of rest. Thyrotoxic periodic paralysis can occur in association with any cause of hyperthyroidism, but is most commonly associated with Graves disease.
    • 序列相似性

      Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1S subfamily.
    • 结构域

      Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      The loop between repeats II and III interacts with the ryanodine receptor, and is therefore important for calcium release from the endoplasmic reticulum necessary for muscle contraction.
    • 翻译后修饰

      Phosphorylation by PKA activates the calcium channel.
    • 细胞定位

      Membrane.
    • Target information above from: UniProt accession Q13698 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 779 Human
      • Omim: 114208 Human
      • SwissProt: Q13698 Human
      • Unigene: 1294 Human
      • 别名

        • alpha-1 polypeptide antibody
        • CAC1S_HUMAN antibody
        • CACH1 antibody
        • Cach1b antibody
        • CACN1 antibody
        • CACNA1S antibody
        • CACNL1A3 antibody
        • Calcium channel antibody
        • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal antibody
        • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle antibody
        • Calcium channel, L type, alpha 1 polypeptide, isoform 3, skeletal muscle, hypokalemic periodic paralysis antibody
        • Calcium channel, skeletal muscle dihydropyridine sensitive , alpha 1 subunit antibody
        • Calcium channel, voltage dependent, L type, alpha 1S subunit antibody
        • Calcium channel, voltage-dependent, L type, alpha 1S subunit, b antibody
        • Cav1.1 antibody
        • CCHL1A3 antibody
        • Dihydropyridine receptor antibody
        • Dihydropyridine sensitive L type calcium channel alpha 1 subunit antibody
        • fmd antibody
        • HOKPP antibody
        • HypoPP antibody
        • isoform 3 antibody
        • L type antibody
        • Malignant hyperthermia susceptibility 5 antibody
        • mdg antibody
        • MHS5 antibody
        • ROB1 antibody
        • sj antibody
        • skeletal muscle antibody
        • TTPP1 antibody
        • Voltage gated calcium channel subunit alpha Cav1.1 antibody
        • Voltage-dependent L-type calcium channel subunit alpha-1S antibody
        • Voltage-gated calcium channel subunit alpha Cav1.1 antibody
        see all

      图片

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1S antibody [1A] (ab2862)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) was performed on normal biopsies of deparaffinized human skeletal muscle tissue. Heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then incubated with ab2862 (1:20) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

      实验方案

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
    • 文献 (12)

      发表研究结果有使用 ab2862?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2862 被引用在 12 文献中.

      • Garibaldi M  et al. 'Dusty core disease' (DuCD): expanding morphological spectrum of RYR1 recessive myopathies. Acta Neuropathol Commun 7:3 (2019). PubMed: 30611313
      • Sébastien M  et al. Deletion of the microtubule-associated protein 6 (MAP6) results in skeletal muscle dysfunction. Skelet Muscle 8:30 (2018). PubMed: 30231928
      • Xiyuan Z  et al. NO-sGC Pathway Modulates Ca2+Release and Muscle Contraction in Zebrafish Skeletal Muscle. Front Physiol 8:607 (2017). PubMed: 28878687
      • Hu H  et al. The molecular architecture of dihydropyrindine receptor/L-type Ca2+ channel complex. Sci Rep 5:8370 (2015). WB . PubMed: 25667046
      • Salanova M  et al. Nitrosative stress in human skeletal muscle attenuated by exercise countermeasure after chronic disuse. Redox Biol 1:514-26 (2013). PubMed: 24251120
      View all Publications for this product

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-7 of 7 Abreviews or Q&A

      Western blot abreview for Anti-CACNA1S antibody [1A]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - other (Skeletal muscle microsomes)
      Gel Running Conditions
      Reduced Denaturing (4–15%)
      Loading amount
      10 µg
      Specification
      Skeletal muscle microsomes
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Abcam user community

      Verified customer

      提交于 Jun 15 2015

      Immunocytochemistry/ Immunofluorescence abreview for Anti-CACNA1S antibody [1A]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Mouse Cell (C2C12 myotube)
      Permeabilization
      Yes - 0.1% triton/PBS
      Specification
      C2C12 myotube
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      提交于 Jun 15 2015

      Question

      What dilution was tested in IP with these antibodies?

      Read More

      Abcam community

      Verified customer

      Asked on Jun 14 2006

      Answer

      Thank you for your enquiry. Regarding the two antibodies, ab2862 and ab2825, I was able to find the specific antibody concentrations for you. The concentration of ab2825 (PMCA ATPase) is 7.7 mg/ml and the concentration of ab2862 (Dihydropyridine Receptor Alpha-1) is 3.8 mg/ml. I would recommend a range for immunoprecipitation assays, 10-500 ug of sample to 0.5-5 ug of antibody. Based on resources I have available, I would recommend starting in the mid-range. I hope this information helps. Please let me know if I can be of further assistance.

      Read More

      Abcam Scientific Support

      回复于 Jun 14 2006

      Question

      I wonder if I can use this antibody for a Western Blot with mouse skeletal muscle TT membranes. Will I have problems because of the anti mouse secondary antibody? What protocol do you recomend me for Western and IP?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 26 2006

      Answer

      Thank you for your enquiry. This antibody has been shown to work by Western blot; detecting a ~200 kDa protein corresponding to the DHP receptor in rat skeletal muscle extracts. You shouldn't have too much of a problem with your IP and western experiments given that IgG migrates at ~50KDa and therefore wont interfere with your targeting of DHPR alpha 1. I would recommend the use of low leaching protein A agarose and localising your blot for all products >100KDa by cutting your membrane with a scalpel. I thought that I would mention that DHPR alpha 1 was detected in skeletal muscle in the following publication. http://www.jbc.org/cgi/reprint/277/52/50535 Zheng Z, Wang ZM, Delbono O. Insulin-like growth factor-1 increases skeletal muscle dihydropyridine receptor alpha 1S transcriptional activity by acting on the cAMP-response element-binding protein element of the promoter region. J Biol Chem. 2002 Dec 27;277(52):50535-42. Epub 2002 Oct 28. Erratum in: J Biol Chem. 2003 May 2;278(18):16453. PMID: 12407098 I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Feb 27 2006

      Question

      Anonymous Abreview submitted on 28 November 2005: DHPR alpha 1 antibody [1A] (ab2862): Sample: Mouse Tissue sections (skeleton muscles) Application: Immunohistochemistry (Frozen sections) Blocking step:: BSA as blocking agent for 1 hour(s)  0 minute(s)  Concentration: 2% Fixation:: Paraformaldehyde Antigen retrieval step:: None Incubation time:: 20 hour(s)  0 minute(s)  Positive control:: none Negative control:: omit the primer antibody. Dilution: 1/200 Rating: Inconclusive Additional notes: It is pity. My tissue sample is from mouse. We are trying to conjugate it. Secondary antibody: Name:: Non-Abcam one was used: Fluorescein (FITC) AffiniPure F(ab')2 Fragment Goa Conjugation: FITC Dilution:: 1/100

      Read More

      Abcam community

      Verified customer

      Asked on Nov 28 2005

      Answer

      Thank you for your enquiry. I am sorry that ab2862 is not working for you. Since you are using the antibody in a tested application for a species that the antibody has been shown to cross-react with, this should work. Without knowing the exact problem you are having, I can only make general suggestions assuming you are seeing no staining. Have you tried blocking with 10% goat serum in place of BSA? You may wish to try this for 30 minutes at room temperature. In addition, you may wish to try incubating with the primary antibody overnight at 4 degrees. If you could give me further details of the protocol you are using by submitting the form below, I would be happy to look into this further and try to help you to get this antibody to work. https://www.abcam.com/index.html?section=ihc&pageconfig=technical&intAbID=2862&mode=questionaire I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

      Read More

      Abcam Scientific Support

      回复于 Nov 28 2005

      Question

      To whom it may concern, In order to make immunoprecipitation experiments with the mAb 1a against L-type calcium channel (DHPR) alpha1s subunit (Catalog ab2862) I need to know the concentration and or the Molecular weight of the Ab. Could you please try to give me some information about that. Sincerely

      Read More

      Abcam community

      Verified customer

      Asked on Jul 27 2004

      Answer

      Thank you for your enquiry. The concentration of ab2862 is 4.4 mg/ml.

      Read More

      Abcam Scientific Support

      回复于 Aug 05 2004

      Question

      Customer would like to know if ab2862 is n- or c-terminal, and if there are any details about it was tested in IHC.

      Read More

      Abcam community

      Verified customer

      Asked on Apr 05 2004

      Answer

      This antibody has not been epitope mapped. The following are references in regards to IHC: Neuron, 5: 339-351, 1990. J. Cell Biol., 123(5): 1161-1174, 1993. Am. J. Physiol., 268(37): F251-F257, 1995. If you have any more questions, please contact us again.

      Read More

      Abcam Scientific Support

      回复于 Apr 07 2004

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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