存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab121659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/250.|
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
- Information by UniProt
- C9orf135 antibody
- CI135_HUMAN antibody
- Uncharacterized protein C9orf135 antibody
Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleoli, nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121659 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Lanes 2-3 : Anti-C9orf135 antibody (ab121659)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing C9orf135 with a C-terminal myc-DDK tag (~3.1kDa)
ab121659, at 1/500 dilution, staining C9orf135 in paraffin-embedded Human stomach tissue by Immunohistochemistry.
ab121659 has not yet been referenced specifically in any publications.