重组Anti-TMEM256抗体[EPR12842] (ab181239)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12842] to TMEM256
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-TMEM256抗体[EPR12842]
参阅全部 TMEM256 一抗 -
描述
兔单克隆抗体[EPR12842] to TMEM256 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human fetal liver, fetal kidney, A549, Y79 and LnCaP lysates; A549 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12842 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab181239于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/200.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
|
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IP |
1/30 - 1/50.
|
说明 |
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Flow Cyt (Intra)
1/200. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa). |
IP
1/30 - 1/50. |
靶标
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相关性
Belongs to the TMEM256 family. -
细胞定位
Membrane; Multi-pass membrane protein -
数据库链接
- Entrez Gene: 254863 Human
- Entrez Gene: 69186 Mouse
- SwissProt: Q8N2U0 Human
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别名
- TMEM256 antibody
- Transmembrane protein 256 antibody
图片
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All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/10000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Y79 cell lysate
Lane 3 : LnCaP cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 12 kDa -
Intracellular flow cytometric analysis of A549 cells (paraformaldehyde-fixed, 2%)labeling TMEM256with ab181239 at 1/200 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab181239 at 1/50 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution. -
All lanes : Anti-TMEM256 antibody [EPR12842] (ab181239) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Predicted band size: 12 kDa
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (0)
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