Anti-c-Myc (phospho S62)抗体(ab51156)
Key features and details
- Rabbit polyclonal to c-Myc (phospho S62)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-c-Myc (phospho S62)抗体
参阅全部 c-Myc 一抗 -
描述
兔多克隆抗体to c-Myc (phospho S62) -
宿主
Rabbit -
特异性
ab51156 detects endogenous levels of Myc only when phosphorylated at serine 62. -
经测试应用
适用于: ICC/IF, WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human c-Myc (N terminal) (phospho S62). The antiserum was produced against synthesized phosphopeptide derived from human Myc around the phosphorylation site of serine 62 (P-L-SP-P-S).
Database link: P01106 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, PBS
PBS without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab51156于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
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IHC-P |
1/50 - 1/100.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa). |
IHC-P
1/50 - 1/100. |
靶标
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功能
Participates in the regulation of gene transcription. Binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes. -
疾病相关
Note=Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors.
Note=A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
Defects in MYC are a cause of Burkitt lymphoma (BL) [MIM:113970]. A form of undifferentiated malignant lymphoma commonly manifested as a large osteolytic lesion in the jaw or as an abdominal mass. Note=Chromosomal aberrations involving MYC are usually found in Burkitt lymphoma. Translocations t(8;14), t(8;22) or t(2;8) which juxtapose MYC to one of the heavy or light chain immunoglobulin gene loci. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain. -
翻译后修饰
Phosphorylated by PRKDC. Phosphorylation at Thr-58 and Ser-62 by GSK3 is required for ubiquitination and degradation by the proteasome.
Ubiquitinated by the SCF(FBXW7) complex when phosphorylated at Thr-58 and Ser-62, leading to its degradation by the proteasome. In the nucleoplasm, ubiquitination is counteracted by USP28, which interacts with isoform 1 of FBXW7 (FBW7alpha), leading to its deubiquitination and preventing degradation. In the nucleolus, however, ubiquitination is not counteracted by USP28, due to the lack of interaction between isoform 4 of FBXW7 (FBW7gamma) and USP28, explaining the selective MYC degradation in the nucleolus. Also polyubiquitinated by the DCX(TRUSS) complex. -
细胞定位
Nucleus > nucleoplasm. Nucleus > nucleolus. - Information by UniProt
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数据库链接
- Entrez Gene: 4609 Human
- Omim: 190080 Human
- SwissProt: P01106 Human
- Unigene: 202453 Human
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形式
c-Myc is also expressed in the cytoplasm. -
别名
- AU016757 antibody
- Avian myelocytomatosis viral oncogene homolog antibody
- bHLHe39 antibody
see all
图片
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All lanes : Anti-c-Myc (phospho S62) antibody (ab51156) at 1/500 dilution
Lane 1 : extracts from 293 cells treated
with 40nM Forskolin for 30min, with phosphopeptide
Lane 2 : extracts from 293 cells treated with 40nM Forskolin for 30min, without phosphopeptide
Predicted band size: 49 kDa
Observed band size: 49 kDa -
Immunohistochemical analysis of c-Myc (phospho S62) expression in paraffin-embedded human breast carcinoma tissue using 1/50 ab51156. left: untreated sample. Right: sample treated with phosphopeptide.
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Immunofluorescence analysis of HeLa cells, treated (left) and untreated (right) with Forskolin (40nM, 15mins), using c-Myc (phospho-Ser62) antibody.
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ICC/IF image of ab51156 stained HepG2 cells. The cells were 4% formlaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51156, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, a goat anti-rabbit DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (36)
ab51156 被引用在 36 文献中.
- Imoto H et al. A text-based computational framework for patient -specific modeling for classification of cancers. iScience 25:103944 (2022). PubMed: 35535207
- Yuan Y et al. PIM1 promotes hepatic conversion by suppressing reprogramming-induced ferroptosis and cell cycle arrest. Nat Commun 13:5237 (2022). PubMed: 36068222
- Al-Qasem AJ et al. Co-targeting CDK2 and CDK4/6 overcomes resistance to aromatase and CDK4/6 inhibitors in ER+ breast cancer. NPJ Precis Oncol 6:68 (2022). PubMed: 36153348
- Zhu Y et al. Significance of serglycin and its binding partners in autocrine promotion of metastasis in esophageal cancer. Theranostics 11:2722-2741 (2021). PubMed: 33456569
- Cao Y et al. Directly targeting c-Myc contributes to the anti-multiple myeloma effect of anlotinib. Cell Death Dis 12:396 (2021). PubMed: 33854043