Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK-293 cells transfected with CACNB4-c-Myc)
Permeabilization
Yes - 0.5% TX-100
Specification
HEK-293 cells transfected with CACNB4-c-Myc
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1xPBS, 0.1% TX 100, 2% goat serum
Dilution
1/250
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: goat anti rabbit 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
HERR DR. Vladimir Milenkovic
Verified customer
提交于 Aug 02 2011