Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Baboon Tissue sections (fetal liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM sodium citrate pH, 6.0
Permeabilization
No
Specification
fetal liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% normal goat serum in PBS
Concentration
0.5 µg/ml
Secondary antibody
Concentration
7 µg/ml
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG (H+L) biotinylated
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Used Image-iT signal enhancer for 30 min before blocking step and Streptavidin/Biotin blocking kit after blocking step as directed, both at RT. Used tertiary detection with Strepavidin-AlexaFluor 488 at 2.0 ug/ml for 30min followed by quenching of autofluorescence with 1% Sudan Black in 70% methanol for 10 min, both at RT. Nuclei were counterstained with 2.0 ug/ml propidium iodide in PBS for 1 min at RT. Image: green=protein, red=propidium iodide-stained nuclei. 400x magnification.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Marcia Burch
Verified customer
提交于 Mar 04 2019