Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - whole cell (EA.hy926 endothelial cells)
Specification
EA.hy926 endothelial cells
Negative control
Igr5 intron 3 contains no c-Jun binding site (Aguilera C et al. Nature. 2011 469: 231-235)
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (final concentrat
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde (final concentrat
Positive control
Position 89340150-89340297 in chromosome 11 has a validated c-Jun site (ENCODE Project Consortium. PLoS Biol. 2011 9:e1001046
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1x ChIP buffer
Additional data
Additional Notes
Image description:
Validation of c-Jun antibody for ChIP. Human EA.hy 926 endothelial cells were treated with scriptaid (5 µM for 24 h). Binding of c-Jun to the indicated DNA regions was studied by ChIP followed by quantitative real-time RT-PCR (qPCR). Columns represent mean ± SEM, n = 3. Igr5 intron 3 contains no c-Jun binding site (Aguilera C et al. Nature. 2011 469: 231-235) and was used as a negative control. Position 89340150-89340297 in chromosome 11 has a validated c-Jun site (ENCODE Project Consortium. PLoS Biol. 2011 9:e1001046) and was used as a positive control.
PubMed ID
22791246: View
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提交于 Jun 05 2013