Key features and details
- Rabbit polyclonal to Bub3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
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纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 40 kDa (predicted molecular weight: 37 kDa).|
功能Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
序列相似性Belongs to the WD repeat BUB3 family.
Contains 5 WD repeats.
细胞定位Nucleus. Chromosome > centromere > kinetochore. Starts to localize at kinetochores in prometaphase I (Pro-MI) stage and maintains the localization until the metaphase I-anaphase I (MI-AI) transition.
- Information by UniProt
- Aa2-050 antibody
- AU019800 antibody
- AU021329 antibody
Anti-Bub3 antibody (ab4180) at 1/500 dilution + HeLa Nuclear Extract at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Western blot of ab4180 on 20ug HeLa Nuclear Extract at 1/500.
Secondary ab: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000 - 1 min exposure)
ab4180 被引用在 6 文献中.
- Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371
- Zhu S et al. A motif from Lys216 to Lys222 in human BUB3 protein is a nuclear localization signal and critical for BUB3 function in mitotic checkpoint. J Biol Chem 290:11282-92 (2015). PubMed: 25814666
- Burns SS et al. Histone deacetylase inhibitor AR-42 differentially affects cell-cycle transit in meningeal and meningioma cells, potently inhibiting NF2-deficient meningioma growth. Cancer Res 73:792-803 (2013). WB . PubMed: 23151902
- Bava FA et al. CPEB1 coordinates alternative 3'-UTR formation with translational regulation. Nature 495:121-5 (2013). PubMed: 23434754
- Golan-Mashiach M et al. Identification of CTCF as a master regulator of the clustered protocadherin genes. Nucleic Acids Res : (2011). EMSA . PubMed: 22210889
- Huang HC et al. Evidence that mitotic exit is a better cancer therapeutic target than spindle assembly. Cancer Cell 16:347-58 (2009). WB ; Human . PubMed: 19800579