Anti-BTN2A2/BTF2抗体[4B11C8] (ab233752)
Key features and details
- Mouse monoclonal [4B11C8] to BTN2A2/BTF2
- Suitable for: ICC/IF, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-BTN2A2/BTF2抗体[4B11C8]
参阅全部 BTN2A2/BTF2 一抗 -
描述
小鼠单克隆抗体[4B11C8] to BTN2A2/BTF2 -
宿主
Mouse -
经测试应用
适用于: ICC/IF, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment corresponding to Human BTN2A2/BTF2 aa 57-237. (Expressed in E.coli).
Database link: Q8WVV5 -
阳性对照
- WB: K562 cell lysate; Human BTN2A2/BTF2 (aa 57-237) recombinant protein; BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
4B11C8 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab233752于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
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WB |
1/500 - 1/2000. Predicted molecular weight: 59 kDa.
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说明 |
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ICC/IF
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. |
WB
1/500 - 1/2000. Predicted molecular weight: 59 kDa. |
靶标
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组织特异性
Highly expressed in brain, bone marrow, small intestine, muscle, spleen and pancreas. Moderate expression was seen in lung, liver and kidney. -
序列相似性
Belongs to the immunoglobulin superfamily. BTN/MOG family.
Contains 1 B30.2/SPRY domain.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 10385 Human
- Omim: 613591 Human
- SwissProt: Q8WVV5 Human
- Unigene: 373938 Human
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别名
- BT2.2 antibody
- BT2A2_HUMAN antibody
- BTF2 antibody
see all
图片
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Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + Human BTN2A2/BTF2 (aa 57-237) recombinant protein
Predicted band size: 59 kDa -
All lanes : Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : BTN2A2/BTF2 (aa 57-237)-hIgGFc transfected HEK-293 cell lysate
Developed using the ECL technique.
Predicted band size: 59 kDa -
Anti-BTN2A2/BTF2 antibody [4B11C8] (ab233752) at 1/500 dilution + K562 cell lysate
Predicted band size: 59 kDa -
Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling BTN2A2/BTF2 with ab233752 at 1/200 dilution (green) compared with a negative control (red).
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HeLa (human prostate adenocarcinoma cell line) cells stained for BTN2A2/BTF2 (green) using ab233752 at 1/200 dilution in ICC/IF.
Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab233752 尚未被引用在任何文献中。