Anti-BSD抗体(ab38307)
Key features and details
- Rabbit polyclonal to BSD
- Suitable for: WB, ELISA, ICC/IF
- Isotype: IgG
概述
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产品名称
Anti-BSD抗体 -
描述
兔多克隆抗体to BSD -
宿主
Rabbit -
特异性
The antibody detects a 13 kDa protein corresponding to the molecular mass of Bsd on SDS-PAGE immunoblots of 293 cells transfected with a lentiviral vector that expresses the Bsd gene product, but is not detected in mock transfected cells. -
经测试应用
适用于: WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Aspergillus terreus -
免疫原
Synthetic peptide corresponding to BSD (N terminal) conjugated to keyhole limpet haemocyanin. Aspergillus terreus
Database link: P0C2P0 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
This antibody was affinity purified using the N-terminal Bsd peptide (without carrier). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab38307于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/1000. Detects a band of approximately 13 kDa.
|
ELISA |
1/2000.
|
|
ICC/IF |
1/100.
|
说明 |
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WB
1/1000. Detects a band of approximately 13 kDa. |
ELISA
1/2000. |
ICC/IF
1/100. |
靶标
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相关性
Retroviral vectors are commonly used for gene expression in vitro and in vivo. The structure of these vectors usually consists of one gene of interest and a selectable marker gene. Fast selection without damaging cells is a critical step for significant gene expression. Blasticidin S deaminase (Bsd) isolated from Bacillus cereus has a neutralizing action on the highly toxic antibiotic blasticidin. A variety of commercially available retroviral vectors, such lentiviral expression systems, utilize Bsd expression for selection using blasticidin. This selectable marker allows selection of stable mammalian cell lines in as little as one week. -
别名
- Blasticidin-S deaminase antibody
- BSD antibody
图片
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All lanes : Anti-BSD antibody (ab38307)
Lane 1 : 293 cells mock transfected
Lanes 2-3 : 293 cells transiently transfected with pLenti6/TR lentiviral vector
Observed band size: 13 kDa why is the actual band size different from the predicted?
Each lane was loaded with 10-20µg total protein. -
ab38307, staining BSD in 293 cells mock transfected (left) and transiently transfected with pLenti6/TR lentiviral vector (right). The cells were fixed in paraformaldehyde (3.75%) for 30 min., then permeabilized in 0.2% Triton X-100 for 15 min. Blocking was done using 1% BSA for 30 min. The primary was used at 1/100 and then an appropriate secondary antibody conjugated to Texas Red was used.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab38307 被引用在 2 文献中.
- Jammart B et al. Very-low-density lipoprotein (VLDL)-producing and hepatitis C virus-replicating HepG2 cells secrete no more lipoviroparticles than VLDL-deficient Huh7.5 cells. J Virol 87:5065-80 (2013). PubMed: 23427158
- de Wispelaere M & Yang PL Mutagenesis of the DI/DIII linker in dengue virus envelope protein impairs viral particle assembly. J Virol 86:7072-83 (2012). PubMed: 22532681