概述

  • 产品名称
  • 描述
    兔多克隆抗体to BRS-3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Human BRS-3 (internal sequence).

  • 阳性对照
    • Extracts from HUVEC cells.
  • 常规说明

    Previously labelled as Bombesin Receptor 3.

性能

应用

Our Abpromise guarantee covers the use of ab75149 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 44 kDa.
ELISA 1/10000.

靶标

  • 功能
    Role in sperm cell division, maturation, or function. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
  • 组织特异性
    In germ cells in testis. Lung carcinoma cells.
  • 序列相似性
    Belongs to the G-protein coupled receptor 1 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BB3 antibody
    • BLP receptor subtype 3 antibody
    • Bombesin like receptor 3 antibody
    • Bombesin Receptor 3 antibody
    • Bombesin receptor subtype 3 antibody
    • Bombesin receptor subtype-3 antibody
    • BRS 3 antibody
    • BRS-3 antibody
    • BRS3 antibody
    • BRS3_HUMAN antibody
    see all

图片

  • All lanes : Anti-BRS-3 antibody (ab75149) at 1/500 dilution

    Lane 1 : Extracts from HUVEC cells
    Lane 2 : Extracts from HUVEC cells with immunising peptide at 5 µg

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 44 kDa
    Observed band size: 47 kDa
    why is the actual band size different from the predicted?

实验方案

文献

ab75149 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-3 of 3 Abreviews or Q&A

Answer

Thank you for contacting us and your interest in our products.


I am pleased to provide the following immunogen information:

The amino acid range of this immunogen is aa180-230 of human Bombesin receptor subtype-3 with accession number of P32247(http://www.uniprot.org/uniprot/P32247). This antibody binds to the extracellular topological domain (based on prediction).


I hope this information has been of help to you. If you have any further questions, please do not hesitate to contact me.

Read More
Application
Western blot
Sample
Mouse Cell lysate - whole cell (STC1)
Loading amount
20 µg
Specification
STC1
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Rob Tolhurst

Verified customer

提交于 Sep 20 2010

Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293, HGT101, CaCo2)
Loading amount
200000 cells
Specification
HEK293, HGT101, CaCo2
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C

Dr. Rob Tolhurst

Verified customer

提交于 Aug 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册