BrdU免疫组化试剂盒(ab125306)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
概述
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产品名称
BrdU免疫组化试剂盒 -
样品类型
Tissue, Adherent cells, Suspension cells -
产品概述
ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: BrdU labeled MCF7 (human breast carcinoma derived) cells grown on glass slides (ab281335).
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说明
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution) is not provided in this kit.
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经测试应用
适用于: IHC-Fr, IHC-Pmore details
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 1 kit 4X Trypsin Enzyme Concentrate 1 x 3ml Blocking Buffer 1 x 6ml Control Slides (stained) 1 slide Control Slides (unstained) 4 slides DAB Solution 1 (DAB Concentrate) 1 x 0.3ml DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml Denaturing Solution 1 x 6ml Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml Hematoxylin Counterstain 1 x 6ml Mounting Media 1 x 6ml Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml Trypsin Dilution Buffer 1 x 12ml -
研究领域
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab125306于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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说明 |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
图片
数据表及文件
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SDS download
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Datasheet download
文献 (21)
ab125306 被引用在 21 文献中.
- Yan Y et al. Adiponectin deficiency promotes endometrial carcinoma pathogenesis and development via activation of mitogen-activated protein kinase. J Pathol 257:146-157 (2022). PubMed: 35072951
- Routledge D et al. The scaffolding protein flot2 promotes cytoneme-based transport of wnt3 in gastric cancer. Elife 11:N/A (2022). PubMed: 36040316
- Park NR et al. Reticulocalbin 3 is involved in postnatal tendon development by regulating collagen fibrillogenesis and cellular maturation. Sci Rep 11:10868 (2021). PubMed: 34035379
- Chou YS et al. G-Protein-Coupled Estrogen Receptor-1 Positively Regulates the Growth Plate Chondrocyte Proliferation in Female Pubertal Mice. Front Cell Dev Biol 9:710664 (2021). PubMed: 34490260
- Suzuki K et al. Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion. J Neuroinflammation 18:86 (2021). PubMed: 33812385