BrdU Cell Proliferation ELISA试剂盒(colorimetric) (ab126556)

概述

  • 产品名称
    BrdU Cell Proliferation ELISA试剂盒(colorimetric)
    参阅全部 BrdU 试剂盒
  • 检测方法
    Colorimetric
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Sandwich (qualitative)
  • 灵敏度
    < 40 cells/well
  • 检测时间
    2h 30m
  • 实验步骤
    Multiple steps standard assay
  • 产品概述

    ab126556 involves incorporation of BrdU into cells cultured in microtiter plates using the cell layer as the solid phase. During the final 2 to 24 hours of culture BrdU is added to wells of the microtiter plate. BrdU will be incorporated into the DNA of dividing cells. To enable antibody binding to the incorporated BrdU cells must be fixed, permeabilized and the DNA denatured. This is all done in one step by treatment with Fixing Solution. Detector anti-BrdU monoclonal antibody is pipetted into the wells and allowed to incubate for one hour, during which time it binds to any incorporated BrdU. Unbound antibody is washed away and horseradish peroxidase-conjugated goat anti-mouse antibody is added, which binds to the Detector Antibody.

    The horseradish peroxidase catalyzes the conversion of the chromogenic substrate tetra-methylbenzidine (TMB) from a colorless solution to a blue solution (or yellow after the addition of stopping reagent), the intensity of which is proportional to the amount of incorporated BrdU in the cells. The colored reaction product is quantified using a spectrophotometer.

    The resultant assay is sensitive, rapid, easy to perform and applicable to high sample throughput. In addition to evaluation of cell proliferation, information such as cell number, morphology and analysis of cellular antigens can be obtained from a single culture.

  • 经测试应用
    适用于: Indirect ELISAmore details
  • 平台
    Microplate

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab126556 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA Use at an assay dependent concentration.

图片

  • Cell proliferation measured in fibroblasts showing cell amounts vs. optical densities

  • Detection of RH7777 (adherent) cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal -to-noise ratio.
  • Detection of RH7777 (adherent) cells per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Detection of Jurkat cells (non-adherent) per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Detection of Jurkat cells (non-adherent) per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Detection of MCF7 cells per well with 2 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.
  • Detection of MCF7 cells per well with 24 hour pulse with BrdU. Y axis - left, OD 450-550 nm. Y axis-right, signal-to-noise ratio.

实验方案

文献

This product has been referenced in:
  • Löfdahl A  et al. Pulmonary fibrosis in vivo displays increased p21 expression reduced by 5-HT2Breceptor antagonists in vitro - a potential pathway affecting proliferation. Sci Rep 8:1927 (2018). Functional Studies ; Human . Read more (PubMed: 29386571) »
  • Guo Y  et al. Protocatechuic acid (PCA) induced a better antiviral effect by immune enhancement in SPF chickens. Microb Pathog 114:233-238 (2018). Chicken . Read more (PubMed: 29217325) »

See all 34 Publications for this product

客户评价及客户问答

The fixative solution provided with BrdU Cell Proliferation ELISA Kit (ab126556) does an excellent job of keeping the cells in the wells.  So it should not be necessary to do anything further to normalize the signal to the number of cells.  

T...

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If we understand your request correctly, you are trying to control to assure an equal number of viable cells is present in each assay well plus and minus BrdU. Our only suggestion would be for you to perform the assay in test tubes or deep well 96-well...

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Q1: Is 20ul Brdu, as described in the protocol, added to each well regardless of whether the well contains 100 or 200ul?
A1: Yes. The amount of Brdu is in excess, so it is fine to add the same 20ul of Brdu whether or not you have added a ...

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Your customer can use this product on rat cells, as it has no species restriction



Yes, ab126556 can be used with pig cells. This kit detects incorporation of BrdU independent of the species of the cell line being tested.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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