Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - PBS + 0.2% Triton X-100 + 4% BSA
Specification
Brain
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 0.2% Triton X-100 + 4% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Donkey anti-Sheep IgG H+L Cross-Adsorbed Secondary
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
I do the following steps for DNA hydrolysis:
1. Incubate sections in pre-warmed 1 M HCL for 30 minutes at 45oC.
2. Neutralize sections by washing three times in PBS for 5 minutes at room temperature.
3. Continue with immunostaining according to standard IHC protocols.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Alexandre Magno
Verified customer
提交于 Aug 02 2018