Anti-BrdU抗体- Proliferation Marker (ab1893)
Key features and details
- Sheep polyclonal to BrdU - Proliferation Marker
- Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-BrdU抗体- Proliferation Marker
参阅全部 BrdU 一抗 -
描述
羊多克隆抗体to BrdU - Proliferation Marker -
宿主
Sheep -
经测试应用
适用于: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
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常规说明
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.60
Constituent: 0.4% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Related Products
应用
Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FrFl | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 10 µg/ml. | |
| IHC-Fr | Use a concentration of 10 µg/ml. | |
| ELISA | Use at an assay dependent concentration. | |
| WB | Use at an assay dependent concentration. |
靶标
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相关性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
细胞定位
Nuclear -
别名
- Bromodeoxyuridine antibody
- BUdr antibody
图片
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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreviewab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
文献 (135)
ab1893 被引用在 135 文献中.
- Cao X et al. Soluble receptor for advanced glycation end-products promotes angiogenesis through activation of STAT3 in myocardial ischemia/reperfusion injury. Apoptosis 25:341-353 (2020). PubMed: 32333220
- Kim CG et al. Effects of Balneotherapy in Jeju Magma-Seawater on Knee Osteoarthritis Model. Sci Rep 10:6620 (2020). PubMed: 32313003
- Liskiewicz A et al. Physical activity reduces anxiety and regulates brain fatty acid synthesis. Mol Brain 13:62 (2020). PubMed: 32303271
- Fu DL et al. Sanhua Decoction, a Classic Herbal Prescription, Exerts Neuroprotection Through Regulating Phosphorylated Tau Level and Promoting Adult Endogenous Neurogenesis After Cerebral Ischemia/Reperfusion Injury. Front Physiol 11:57 (2020). PubMed: 32116767
- Ostasov P et al. Sonic Hedgehog and Triiodothyronine Pathway Interact in Mouse Embryonic Neural Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32456161
