Anti-BrdU抗体- Proliferation Marker (ab1893)
Key features and details
- Sheep polyclonal to BrdU - Proliferation Marker
- Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WB
- Reacts with: Species independent
- Isotype: IgG
概述
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产品名称
Anti-BrdU抗体- Proliferation Marker
参阅全部 BrdU 一抗 -
描述
羊多克隆抗体to BrdU - Proliferation Marker -
宿主
Sheep -
经测试应用
适用于: IHC-FrFl, IHC-P, IHC-Fr, ELISA, WBmore details -
种属反应性
与反应: Species independent -
免疫原
Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)
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常规说明
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.60
Constituent: 0.4% PBS -
Concentration information loading... -
纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Isotype control
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Related Products
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-FrFl | (1) |
Use at an assay dependent concentration.
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| IHC-P | (7) |
Use a concentration of 10 µg/ml.
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| IHC-Fr | (2) |
Use a concentration of 10 µg/ml.
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| ELISA |
Use at an assay dependent concentration.
|
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| WB |
Use at an assay dependent concentration.
|
| 说明 |
|---|
|
IHC-FrFl
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 10 µg/ml. |
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IHC-Fr
Use a concentration of 10 µg/ml. |
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ELISA
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. |
靶标
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相关性
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
细胞定位
Nuclear -
别名
- Bromodeoxyuridine antibody
- BUdr antibody
图片
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Immunohistochemistry (Frozen sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)Pfeiffer et al PLoS One. 2018 Mar 28;13(3):e0192067. doi: 10.1371/journal.pone.0192067. eCollection 2018. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody - Proliferation Marker (ab1893)This image is courtesy of an anonymous Abreviewab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (135)
ab1893 被引用在 135 文献中.
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- Fu DL et al. Sanhua Decoction, a Classic Herbal Prescription, Exerts Neuroprotection Through Regulating Phosphorylated Tau Level and Promoting Adult Endogenous Neurogenesis After Cerebral Ischemia/Reperfusion Injury. Front Physiol 11:57 (2020). PubMed: 32116767
- Ostasov P et al. Sonic Hedgehog and Triiodothyronine Pathway Interact in Mouse Embryonic Neural Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32456161