Application
Immunocytochemistry/ Immunofluorescence
Sample
African Green Monkey Cell (Oligodendrocytes, astrocytes (mixed glial culture))
Specification
Oligodendrocytes, astrocytes (mixed glial culture)
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-PBS for 30 minutes followed by DNAse treatment for 90 minutes.
Other product details
Incubation time
0 minute(s) · Temperature: 4°C · Diluent: 10% Goat serum in 0.3% Triton-PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Mixed primary glial cell culture (rat).
Purple: BrdU
Staining colocalizes with nucleus (DAPI) as expected. Very specific. Little/no background.
Purple: BrdU
Staining colocalizes with nucleus (DAPI) as expected. Very specific. Little/no background.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 28 2010