BrdU (5-bromo-2'-deoxyuridine),Thymidine analog (ab142567)
Key features and details
- Thymidine analog for measuring cell proliferation
- CAS Number: 59-14-3
- Purity: > 98%
- Soluble in water to 50 mM
- Form / State: Solid
- Source: Synthetic
概述
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产品名称
BrdU (5-bromo-2'-deoxyuridine),Thymidine analog -
描述
Thymidine analog for measuring cell proliferation -
纯度
> 98% -
CAS编号
59-14-3 -
化学结构
性能
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化学名称
5-Bromo-1-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione -
分子量
307.10 -
分子式
C9H11BrN2O5 -
PubChem识别号
6035 -
存放说明
Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解度概述
Soluble in water to 50 mM -
处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Toxic, refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
BrC1=CN(C(=O)NC1=O)[C@H]2CC(O)[C@@H](CO)O2 -
来源
Synthetic
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研究领域
相关产品
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图片
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ab220075 staining BrdU in HeLa cells. Untreated and BrdU treated (ab142567, 10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab220075 at 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in HeLa cells fixed with 4% formaldehyde (10 min).
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2D chemical structure image of ab142567, BrdU (5-bromo-2'-deoxyuridine), Thymidine analog
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Estimation of S-phase Duration using EdU-BrdU Dual Pulse Labelling.
(From Figure 5A of Massey)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
文献 (69)
ab142567 被引用在 69 文献中.
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- Mathiesen A et al. Adipose Tissue-Derived Extracellular Vesicles Contribute to Phenotypic Plasticity of Prostate Cancer Cells. Int J Mol Sci 24:N/A (2023). PubMed: 36674745
- Feng R et al. Self-renewing macrophages in dorsal root ganglia contribute to promote nerve regeneration. Proc Natl Acad Sci U S A 120:e2215906120 (2023). PubMed: 36763532
- Fujii Y et al. Rhodopsin-positive cell production by intravitreal injection of small molecule compounds in mouse models of retinal degeneration. PLoS One 18:e0282174 (2023). PubMed: 36821627