Anti-BRD8/p120抗体(ab17969)
Key features and details
- Rabbit polyclonal to BRD8/p120
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BRD8/p120抗体
参阅全部 BRD8/p120 一抗 -
描述
兔多克隆抗体to BRD8/p120 -
宿主
Rabbit -
特异性
Specific to p120 -
经测试应用
适用于: IP, IHC-P, WBmore details -
种属反应性
与反应: Human
预测可用于: Rat, Rabbit, Horse, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla, Opossum, Chinese hamster, Elephant -
免疫原
Synthetic peptide within BRD8/p120 aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9H0E9 -
阳性对照
- Nuclear extract from HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Antibody was affinity purified using an epitope specific to BRD8/p120 immobilized on solid support. -
克隆
多克隆 -
同种型
IgG -
研究领域
- Signal Transduction
- Signaling Pathway
- Nuclear Signaling
- Nuclear Hormone Receptors
- Co-activators/co-repressors
- Epigenetics and Nuclear Signaling
- Transcription
- Polymerase associated factors
- Pol II Transcription
- Other
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab17969于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa).
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说明 |
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IP
Use at an assay dependent concentration. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/10000 - 1/25000. Detects a band of approximately 170 kDa (predicted molecular weight: 138 kDa). |
靶标
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功能
May act as a coactivator during transcriptional activation by hormone-activated nuclear receptors (NR). Isoform 2 stimulates transcriptional activation by AR/DHTR, ESR1/NR3A1, RXRA/NR2B1 and THRB/ERBA2. At least isoform 1 and isoform 2 are components of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage. Component of a SWR1-like complex that specifically mediates the removal of histone H2A.Z/H2AFZ from the nucleosome. -
组织特异性
Expressed in adipose tissue, brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
序列相似性
Contains 2 bromo domains. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10902 Human
- Entrez Gene: 291691 Rat
- Omim: 602848 Human
- SwissProt: Q9H0E9 Human
- SwissProt: Q5TLG7 Rat
- Unigene: 519337 Human
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别名
- Brd8 antibody
- BRD8_HUMAN antibody
- Bromodomain containing 8 antibody
see all
图片
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ab17969 immunoprecipitating BRD8 at 6 µg per reaction.
Lane 1: ab17969 in HeLa nuclear extract (0.5 or 1 mg per IP reaction; 20% of IP loaded).
Lane 2: IgG control
For blotting immunoprecipitated BRD8, ab17969 was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRD8 / p120 with ab17969 at 1/1000 (1µg/ml). Detection: DAB.
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Detection of human BRD8 by Western Blot. Sample is nuclear
extract (NE; 5, 15, and 50mcg) from HeLa cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab17969 被引用在 2 文献中.
- Couture JP et al. The p400/Brd8 chromatin remodeling complex promotes adipogenesis by incorporating histone variant H2A.Z at PPAR? target genes. Endocrinology 153:5796-808 (2012). PubMed: 23064015
- Yamaguchi K et al. C20orf20 (MRG-binding protein) as a potential therapeutic target for colorectal cancer. Br J Cancer 102:325-31 (2010). WB, IP ; Human . PubMed: 20051959