Anti-BRD2抗体(ab3718)
Key features and details
- Goat polyclonal to BRD2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BRD2抗体
参阅全部 BRD2 一抗 -
描述
山羊多克隆抗体to BRD2 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Cow, Rhesus monkey, Gorilla
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免疫原
Synthetic peptide (Human) - which represented a portion of human Bromodomain Containing 2 (BRD 2) between the putative nuclear localization signal and the ET domain.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
Our Abpromise guarantee covers the use of ab3718 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/10000 - 1/40000. Predicted molecular weight: 110 kDa. |
靶标
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功能
May play a role in spermatogenesis or folliculogenesis. -
序列相似性
Contains 2 bromo domains.
Contains 1 ET domain. -
结构域
One bromodomain is sufficient for a partial interaction with histone H4 acetylated at 'Lys-13'. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 505358 Cow
- Entrez Gene: 6046 Human
- Entrez Gene: 14312 Mouse
- Omim: 601540 Human
- SwissProt: Q32S26 Cow
- SwissProt: P25440 Human
- SwissProt: Q7JJ13 Mouse
- Unigene: 75243 Human
see all -
别名
- BRD 2 antibody
- Brd2 antibody
- BRD2_HUMAN antibody
see all
图片
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Western blot - Anti-BRD2 antibody (ab3718)
Sample: Hela cell total RIPA extract (10
µ g)Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000)
CIP: Calf Intestinal Phosphatase
Detection: ECL exposed for 1 minute.
Sample: Hela cell total RIPA extract (10µg) Antibody: Affinity purified Goat anti-BRD 2 (ab3718) (diluted 1 / 20,000) CIP: Calf Intestinal Phosphatase Detection: ECL exposed for 1 minute.
实验方案
文献 (6)
ab3718 被引用在 6 文献中.
- Khan KA et al. The Use of Mononucleosome Immunoprecipitation for Analysis of Combinatorial Histone Post-translational Modifications and Purification of Nucleosome-Interacting Proteins. Front Cell Dev Biol 8:331 (2020). PubMed: 32457909
- Sun Y et al. BET protein inhibition mitigates acute myocardial infarction damage in rats via the TLR4/TRAF6/NF-?B pathway. Exp Ther Med 10:2319-2324 (2015). WB . PubMed: 26668635
- Dhar S et al. Insights into role of bromodomain, testis-specific (Brdt) in acetylated histone H4-dependent chromatin remodeling in mammalian spermiogenesis. J Biol Chem 287:6387-405 (2012). ICC/IF ; Rat . PubMed: 22215678
- Draker R et al. A combination of H2A.Z and H4 acetylation recruits Brd2 to chromatin during transcriptional activation. PLoS Genet 8:e1003047 (2012). WB . PubMed: 23144632
- Lin A et al. The EBNA1 protein of Epstein-Barr virus functionally interacts with Brd4. J Virol 82:12009-19 (2008). WB ; Human . PubMed: 18922874
- Benevolenskaya EV et al. Binding of pRB to the PHD protein RBP2 promotes cellular differentiation. Mol Cell 18:623-35 (2005). PubMed: 15949438
