为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation

Anti-BRCA1 (phospho S1524)抗体(ab2401)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-BRCA1 (phospho S1524) antibody (ab2401)

    Key features and details

    • Rabbit polyclonal to BRCA1 (phospho S1524)
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    你可能会感兴趣

    二抗
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    蛋白
    Product image
    Recombinant Human BRCA1 protein (ab82204)

    查看更多相关产品

    概述

    • 产品名称

      Anti-BRCA1 (phospho S1524)抗体
      参阅全部 BRCA1 一抗
    • 描述

      兔多克隆抗体to BRCA1 (phospho S1524)
    • 宿主

      Rabbit
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Human
      预测可用于: Chimpanzee, Gorilla
    • 免疫原

      Synthetic phospho peptide (Human) - which represents a portion of human Breast Cancer Gene 1 around serine 1524.

    • 阳性对照

      • HeLa cells treated with IR.
    • 常规说明

      LocusLink ID 672.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Antibodies that were not phospho-specific were removed by solid phase absorption. Antibodies specific for BRCA1 pSer1524 were affinity purified using the phosphopeptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Ubiquitylation
      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • DNA Damage Response
      • BRCA1
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • RING Finger E3 Ligase
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Cancer
      • Oncoproteins/suppressors
      • Tumor suppressors
      • Other

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human BRCA1 protein (ab82204)

    应用

    Our Abpromise guarantee covers the use of ab2401 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/500 - 1/5000. Detects a band of approximately 210 kDa (predicted molecular weight: 208 kDa).

    靶标

    • 功能

      E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks.
    • 组织特异性

      Isoform 1 and isoform 3 are widely expressed. Isoform 3 is reduced or absent in several breast and ovarian cancer cell lines.
    • 通路

      Protein modification; protein ubiquitination.
    • 疾病相关

      Defects in BRCA1 are a cause of susceptibility to breast cancer (BC) [MIM:114480]. A common malignancy originating from breast epithelial tissue. Breast neoplasms can be distinguished by their histologic pattern. Invasive ductal carcinoma is by far the most common type. Breast cancer is etiologically and genetically heterogeneous. Important genetic factors have been indicated by familial occurrence and bilateral involvement. Mutations at more than one locus can be involved in different families or even in the same case. Note=Mutations in BRCA1 are thought to be responsible for 45% of inherited breast cancer. Moreover, BRCA1 carriers have a 4-fold increased risk of colon cancer, whereas male carriers face a 3-fold increased risk of prostate cancer. Cells lacking BRCA1 show defects in DNA repair by homologous recombination.
      Defects in BRCA1 are a cause of susceptibility to breast-ovarian cancer familial type 1 (BROVCA1) [MIM:604370]. A condition associated with familial predisposition to cancer of the breast and ovaries. Characteristic features in affected families are an early age of onset of breast cancer (often before age 50), increased chance of bilateral cancers (cancer that develop in both breasts, or both ovaries, independently), frequent occurrence of breast cancer among men, increased incidence of tumors of other specific organs, such as the prostate. Note=Mutations in BRCA1 are thought to be responsible for more than 80% of inherited breast-ovarian cancer.
      Defects in BRCA1 are a cause of genetic susceptibility to ovarian cancer [MIM:113705].
    • 序列相似性

      Contains 2 BRCT domains.
      Contains 1 RING-type zinc finger.
    • 结构域

      The BRCT domains recognize and bind phosphorylated pSXXF motif on proteins. The interaction with the phosphorylated pSXXF motif of FAM175A/Abraxas, recruits BRCA1 at DNA damage sites.
      The RING-type zinc finger domain interacts with BAP1.
    • 翻译后修饰

      Phosphorylation at Ser-308 by STK6/AURKA is required for normal cell cycle progression from G2 to mitosis. Phosphorylated in response to IR, UV, and various stimuli that cause checkpoint activation, probably by ATM or ATR.
      Autoubiquitinated, undergoes 'Lys-6'-linked polyubiquitination. 'Lys-6'-linked polyubiquitination does not promote degradation.
    • 细胞定位

      Cytoplasm; Nucleus. Localizes at sites of DNA damage at double-strand breaks (DSBs) and recruitment to DNA damage sites is mediated by the BRCA1-A complex.
    • Target information above from: UniProt accession P38398 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 672 Human
      • Omim: 113705 Human
      • SwissProt: Q9GKK8 Chimpanzee
      • SwissProt: Q6J6I8 Gorilla
      • SwissProt: P38398 Human
      • Unigene: 194143 Human
      • 别名

        • BRCA 1 antibody
        • BRCA1 antibody
        • BRCA1 DNA repair associated antibody
        • BRCA1/BRCA2 containing complex subunit 1 antibody
        • BRCA1/BRCA2-containing complex, subunit 1 antibody
        • BRCA1_HUMAN antibody
        • BRCAI antibody
        • BRCC 1 antibody
        • BRCC1 antibody
        • Breast and ovarian cancer susceptibility protein 1 antibody
        • Breast Cancer 1 antibody
        • Breast Cancer 1 Early Onset antibody
        • Breast cancer type 1 susceptibility protein antibody
        • BROVCA1 antibody
        • FANCS antibody
        • IRIS antibody
        • PNCA4 antibody
        • PPP1R53 antibody
        • Protein phosphatase 1 regulatory subunit 53 antibody
        • PSCP antibody
        • RING finger protein 53 antibody
        • RNF53 antibody
        see all

      图片

      • Western blot - Anti-BRCA1 (phospho S1524) antibody (ab2401)
        Western blot - Anti-BRCA1 (phospho S1524) antibody (ab2401)

        Rabbit polyclonal to phospho BRCA1 (Ser1524) used at 1/2000.

        Samples: Nuclear extract from HeLa cells, untreated (-) or treated (+) with ionising radiation (IR).



        Rabbit polyclonal to phospho BRCA1 (Ser1524) used at 1/2000. Samples: Nuclear extract from HeLa cells, untreated (-) or treated (+) with ionising radiation (IR).

      实验方案

      • Western blot protocols

      Click here to view the general protocols

      数据表及文件

      • Datasheet
      • SDS
    • 文献 (1)

      发表研究结果有使用 ab2401?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab2401 被引用在 1 文献中.

      • Burdova K  et al. WIP1 Promotes Homologous Recombination and Modulates Sensitivity to PARP Inhibitors. Cells 8:N/A (2019). PubMed: 31619012

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Western blot abreview for Anti-BRCA1 (phospho S1524) antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (293T Cells)
      Loading amount
      30 µg
      Specification
      293T Cells
      Treatment
      10uM Bleomycin for 30 min.
      Gel Running Conditions
      Reduced Denaturing (6% Acrylamide Gel)
      Blocking step
      Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      提交于 Nov 18 2009

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      通过邮件收到学术资源和促销信息 注册
      研究类别(A-Z)
      • 肿瘤研究
      • 心血管研究
      • 细胞生物学
      • 发育生物学
      • 染色质及细胞核信号转导研究
      • 免疫学
      • 代谢
      • 微生物学
      • 神经生物学
      • 信号转导研究
      • 干细胞研究
      产品种类(A-Z)
      • 一抗
      • 二抗
      • 生化试剂
      • 亚类对照
      • 流式细胞仪多色选择器
      • 试剂盒
      • 内参对照
      • 细胞裂解液
      • 多肽
      • 蛋白酶
      • 组织切片
      • 蛋白标签及细胞标记物
      • 相关试剂及工具
      帮助与支持
      • 技术支持
      • 技术FAQ
      • 实验步骤与建议
      • 购买FAQ
      • RabMAb 产品
      • 培训课程
      • 按靶标浏览
      企业
      • 企业资讯
      • 投资者关系
      • 公司新闻
      • 招贤纳士
      • 关于我们
      活动通知
      • 商品展览
      • 会议
      全球网站
      • abcam.com
      • abcam.co.jp
      QR code for Weechat

      微信扫码 关注我们

      ?ICP?14046373?-1

      ????? 31011502007413?

      • 销售条款
      • 网站使用条款
      • Cookie政策
      • 隐私权政策
      • 法律
      © 1998-2021 艾博抗(上海)贸易有限公司版权所有