Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Human Cell lysate - whole cell (Hela cells)
Specification
Hela cells
Total protein in input
2000 µg
Other product details
Concentration
1 µg/mg lysate
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 50mM TRis, 150 NaCl, 0.5% Igepal-630
Western Blot antibody
Name
Non-Abcam antibody was used: EMD Millipore
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Remi Buisson
Verified customer
提交于 Jan 05 2015