Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (6%)
Sample
Human Cell lysate - whole cell (U2OS osteosarcoma)
Specification
U2OS osteosarcoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Li-CoR Donkey anti Mouse Antibody
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 680LT
Host species: Donkey
Clonality: Monoclonal
Conjugation: IRDye 680LT
Dilution
1/15000
Detection
Detection method
Li-Cor Odyssey Scanner
Bands
Specific: 200 kDa Non-specific: 300 kDa
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
Specific band observed around 220kD, but prominent smear observed. It is known that BRCA1 is heavily modified by various small modifiers so it is unsure whether these bands are specific.
1. undamaged sample
2. endonuclease induced damage, 30 mins
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 25 2014