Application
Western blot
Sample
Human Cell lysate - whole cell (melanoma cell lines)
Loading amount
50 µg
Specification
melanoma cell lines
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS,0.02% Tween
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 87 kDa
Additional data
Additional Notes
weak but specific band for G361, stronger signal under reducing conditions
G361 and A2058 are human melanoma cell lines
G361 and A2058 are human melanoma cell lines
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 02 2010