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Epigenetics and Nuclear Signaling Transcription Cancer susceptibility Tumor Suppressors

Anti-BPOZ/ABTB1抗体(ab1077)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

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Western blot - Anti-BPOZ/ABTB1 antibody (ab1077)

    Key features and details

    • Goat polyclonal to BPOZ/ABTB1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-BPOZ/ABTB1抗体
    • 描述

      山羊多克隆抗体to BPOZ/ABTB1
    • 宿主

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • 免疫原

      Synthetic peptide corresponding to Human BPOZ/ABTB1 aa 323-336 (N terminal).
      Sequence:

      RALEDLLVSIGLDC

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 常规说明

       This product was previously labelled as BPOZ

       

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • 存储溶液

      pH: 7.30
      Constituent: 0.5% BSA
    • Concentration information loading...
    • 纯度

      Immunogen affinity purified
    • 纯化说明

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Cancer susceptibility
      • Tumor Suppressors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Other factors
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Elongation factors

    相关产品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab1077 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Human
    应用 Ab评论 说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

    1 hour primary incubation is recommended for this product.

    说明
    WB
    Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).

    1 hour primary incubation is recommended for this product.

    靶标

    • 功能

      May act as a mediator of the PTEN growth-suppressive signaling pathway. May play a role in developmental processes.
    • 组织特异性

      Ubiquitously expressed in all fetal tissues examined including heart, brain, liver, and kidney. Also expressed at lower levels in both adult heart and hypertrophic heart.
    • 序列相似性

      Contains 2 ANK repeats.
      Contains 2 BTB (POZ) domains.
    • 细胞定位

      Cytoplasm.
    • Target information above from: UniProt accession Q969K4 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 80325 Human
      • Omim: 608308 Human
      • SwissProt: Q969K4 Human
      • Unigene: 107812 Human
      • 别名

        • ABTB 1 antibody
        • Abtb1 antibody
        • ABTB1_HUMAN antibody
        • Ankyrin repeat and BTB (POZ) domain containing 1 antibody
        • Ankyrin repeat and BTB/POZ domain containing 1 antibody
        • Ankyrin repeat and BTB/POZ domain containing protein 1 antibody
        • Ankyrin repeat and BTB/POZ domain-containing protein 1 antibody
        • EF1ABP antibody
        • Elongation factor 1A binding protein antibody
        • Elongation factor 1A-binding protein antibody
        • MGC20585 antibody
        • PP 2259 antibody
        • PP2259 antibody
        see all

      图片

      • Western blot - Anti-BPOZ/ABTB1 antibody (ab1077)
        Western blot - Anti-BPOZ/ABTB1 antibody (ab1077)

        ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa  BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

        ab1077 at a concentration of 0.2µg/ml staining approximately 40kDa BPOZ/ABTB1 in human brain extract (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      实验方案

      • Western blot protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      数据表及文件

      • SDS download

      • Datasheet download

        Download

      文献 (1)

      发表研究结果有使用 ab1077?请让我们知道,以便我们可以引用本数据表中的参考文章。

      ab1077 被引用在 1 文献中.

      • Roy A & Pahan K Ankyrin repeat and BTB/POZ domain containing protein-2 inhibits the aggregation of alpha-synuclein: Implications for Parkinson's disease. FEBS Lett 587:3567-74 (2013). ICC/IF ; Mouse . PubMed: 24076025

      客户评价及客户问答

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      There are currently no Customer reviews or Questions for ab1077.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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