重组Anti-BPI抗体[EPR12350(B)] (ab175231)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12350(B)] to BPI
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-BPI抗体[EPR12350(B)]
参阅全部 BPI 一抗 -
描述
兔单克隆抗体[EPR12350(B)] to BPI -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P or IP -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
Synthetic peptide within Human BPI aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: P17213 -
阳性对照
- Human bone marrow, THP1 and HL60 lysates; HL60 cells; THP1 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
EPR12350(B) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175231于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB |
1/1000 - 1/5000. Predicted molecular weight: 54 kDa.
|
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ICC/IF |
1/100 - 1/250.
|
说明 |
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Flow Cyt (Intra)
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/5000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100 - 1/250. |
靶标
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相关性
The antimicrobial protein Bactericidal Permeability Increasing protein (BPI) is a 55 kDa protein found in the primary granules of polymorhonuclear leukocytes (PMN). The cytotoxicity action of BPI is limited to gram negative bacteria, reflecting the high affinity of BPI for bacterial LPS. Binding of BPI to live bacteria via LPS causes anti-infective activites: 1) cytotoxicity via sequential damage to bacterial outer and inner lipid membranes, 2) neutralization of gram-negative bacterial LPS, 3) opsonization of bacteria to enhance phagocytosis by neutrophils. -
细胞定位
Cell Membrane and Secreted. Membrane associated in polymorphonuclear Leukocytes (PMN) granules. -
数据库链接
- Entrez Gene: 671 Human
- Omim: 109195 Human
- SwissProt: P17213 Human
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别名
- Bactericidal permeability-increasing protein antibody
- bactericidal/permeability-increasing protein antibody
- BPI antibody
see all
图片
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Immunofluorescent analysis of HL60 cells labeling BPI with ab175231 at 1/100 (red) and DAPI staining (blue).
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All lanes : Anti-BPI antibody [EPR12350(B)] (ab175231) at 1/1000 dilution
Lane 1 : Human bone marrow lysate
Lane 2 : THP1 lysate
Lane 3 : HL60 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Additional bands at: 50-60 kDa. We are unsure as to the identity of these extra bands. -
Intracellular flow cytometrical analysis of permeabilized THP1 cells labeling BPI with ab175231 antibody at 1/100 (red) or a rabbit IgG (green).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab175231 尚未被引用在任何文献中。