Anti-Bovine Serum Albumin抗体[BSA-7G10] (ab3781)

概述

  • 产品名称
    Anti-Bovine Serum Albumin抗体[BSA-7G10]
    参阅全部 Bovine Serum Albumin 一抗
  • 描述
    小鼠单克隆抗体[BSA-7G10] to Bovine Serum Albumin
  • 宿主
    Mouse
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow
    预测可用于: Rabbit, Human
  • 免疫原

    Full length native protein (purified) (Cow).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Constituents: 1.2114% Tris, 0.7507% Glycine, 2% Sucrose
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    BSA-7G10
  • 骨髓瘤
    unknown
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab3781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000 - 1/10000.
WB 1/1200 - 1/2000.

靶标

实验方案

文献

This product has been referenced in:
  • Walkup WG  et al. Phosphorylation of synaptic GTPase-activating protein (synGAP) by Ca2+/calmodulin-dependent protein kinase II (CaMKII) and cyclin-dependent kinase 5 (CDK5) alters the ratio of its GAP activity toward Ras and Rap GTPases. J Biol Chem 290:4908-27 (2015). WB . Read more (PubMed: 25533468) »
  • Wagner CS  et al. Intracellular regulation of cross-presentation during dendritic cell maturation. PLoS One 8:e76801 (2013). Read more (PubMed: 24098562) »
See all 3 Publications for this product

客户评价及客户问答

1-7 of 7 Q&A

Answer

Thank you for your reply.

I heard back from the lab that ab3781 can be used with this standard protocol:
- Blocking for 20 min to 1 hour with TBST 1X with 5% dry milk
- Wash briefly with TBST 1X
- Incubation with the antibody at a dilution between 1/1200 to 1/2000 for 1 to 4 hours at room temperature
- Wash with TBST 1X once briefly and 5 times for 5 min
Then incubate with the secondary antibody acourding manufacturer instructions.

I hope this information helps. If you are still experiencing problems with the antibody, please let me know and we would be happy to replace or refund the antibody for you.

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Answer

Thank you for your inquiry.

I heard back from the lab that this antibody has been successfully tested on BSA in WB. The blocking buffer was TBS-Tween 20 with 5% dry milk. Is this what you had tried?

What dilutions of the antibody have you tried? Plus were you incubating overnight at 4C?

I look forward to your reply.

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Question
Answer

Thank you for contacting us.

Please type Bovine Serum Album in the search box or click on the following link

https://www.abcam.com/index.html?pageconfig=searchresults

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your phone call.
I am very sorry to hear that these antibodies did not work together in sandwich ELISA. I am sending you the following discount codes as compensation for trying the antibodies in an untested application. Unfortunately, our guarantee does not apply in this case.
Please let me know the other 2 anti-BSA antibodies that you would like to try together in sandwich ELISA and I'd be happy to issue you the testing discount codes as well.
DISCOUNT CODE: xxx for ab3781
DISCOUNT CODE: xxx for ab7637
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab3781 and ab7637 in sandwich ELISA. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for sandwich ELISA and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us. These two anti-BSA antibodies are provided as ascites. Monoclonal antibodies can be produced by growing hybridoma cells within the peritoneal cavity of a mouse. When injected into a mouse, the hybridoma cells multiply and produce fluid (ascites) in its abdomen. This fluid contains a high concentration of antibody which can be harvested. This usually provides higher antibody yields than hybridoma cell culture. Unfortunately, the concentration of the specific antibody cannot be determined as there are many proteins present in ascites. Furthermore, these antibodies are not further purified from the fluid harvested and thus no particular storage buffer is added. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry. Unfortunately, epitope mapping was not done on this product, therefore we do not have any information on the exact epitope binding region. I am sorry I can't be of more assistance in this occasion but if there is anything else that I can help you with, please do not hesitate to contact me.

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Question
Answer

This antibody is only available in liquid form.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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