Bovine Heart Mitochondria (ab110338)
概述
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产品名称
Bovine Heart Mitochondria -
常规说明
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols. -
经测试应用
适用于: IP, SDS-PAGE, WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.02% PMSF, 0.16% Tris, 0.89% EDTA, 8.56% Sucrose -
Concentration information loading...
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裂解物说明
2 mg of bovine heart mitochondria in iso-osmotic buffer. This sample can act as a positive control in immunocapture applications and Blue Native PAGE.
1 mg solubilized mitochondria for Immunocapture experiments. For the Immunocapture of OXPHOS complexes, it is recommended that 180 µl of the mitochondrial membranes supplied be solubilized by the addition of 20 µl of 10% lauryl maltoside. Insoluble material is removed by centrifugation at 72,000g before immunocapture of complexes from the supernatant. For more detail see Abcam's suggested protocols.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110338于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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SDS-PAGE |
Use at an assay dependent concentration.
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|
WB |
Use at an assay dependent concentration.
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说明 |
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IP
Use at an assay dependent concentration. |
SDS-PAGE
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab110338 被引用在 3 文献中.
- Andreux PA et al. Mitochondrial function is impaired in the skeletal muscle of pre-frail elderly. Sci Rep 8:8548 (2018). PubMed: 29867098
- Gómez-Serrano M et al. Differential proteomic and oxidative profiles unveil dysfunctional protein import to adipocyte mitochondria in obesity-associated aging and diabetes. Redox Biol 11:415-428 (2017). Functional Studies ; Human . PubMed: 28064117
- Shell JR & Lawrence DS Proteolytic regulation of the mitochondrial cAMP-dependent protein kinase. Biochemistry 51:2258-64 (2012). PubMed: 22385295