概述

  • 产品名称
  • 描述
    兔多克隆抗体to BOD1L
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen: PQVEKAVHEF LATLNHKEEG SGNTAPDDEK PDTSLITQGV PTPGPSANVA NDAMSILETI TSLNQEASAA RASTETSNAK TSE, corresponding to amino acids 149-231 of Human BOD1L according to Q8NFC6.

  • 阳性对照
    • Human placenta tissue.

性能

应用

Our Abpromise guarantee covers the use of ab122380 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 序列相似性
    Belongs to the BOD1 family.
    Contains 1 A.T hook DNA-binding domain.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Biorientation of chromosomes in cell division 1 like antibody
    • Biorientation of chromosomes in cell division protein 1-like antibody
    • BOD1L antibody
    • BOD1L_HUMAN antibody
    • FAM44A antibody
    • Family with sequence similarity 44, member A antibody
    • Protein FAM44A antibody
    see all

图片

  • Immunofluorescent staining of Human cell line U-251 MG shows positivity in nucleus but not nucleoli. Recommended concentration of ab122380 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122380 at 1/15 dilution staining BOD1L in paraffin-embedded Human placenta tissue by Immunohistochemistry.

文献

ab122380 has not yet been referenced specifically in any publications.

客户评价及客户问答

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Formaldehyde

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提交于 Jul 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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