Anti-BOB1抗体(ab3426)

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Western blot - Anti-BOB1 antibody (ab3426)

    Key features and details

    • Goat polyclonal to BOB1
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    重组抗体的批间可重复性更强

    Product image
    Anti-BOB1 antibody [EP3300] (ab76010)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-BOB1抗体
      参阅全部 BOB1 一抗

    • 描述

      山羊多克隆抗体to BOB1

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Mouse
      预测可用于: Human

    • 免疫原

      Synthetic peptide: DAYALNHTLSVEGF, corresponding to C terminal amino acids 243-256 of Human BOB1.

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 阳性对照

      • Mouse spleen lysate.

    • 常规说明

      Principal Names – POU2AF1; BOB1; OBF1; OCAB; OBF-1; POU domain, class 2, associating factor 1. Official Gene Symbol - POU2AF1. GenBank Accession Number – NP_006226. LocusLink ID - 5450 (human); 18985 (mouse). Gene Ontology terms - transcription cofactor activity; transcription co-activator activity; transcription activating factor; humoral immune response; transcription from Pol II promoter.

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      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab3426 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

    A 1 hour primary incubation is recommended for this product. Detects a band of approximately 30 kDa in Mouse Spleen lysate.

    靶标

    • 功能

      Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

    • 组织特异性

      B-cell specific.

    • 疾病相关

      Note=A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6.

    • 序列相似性

      Belongs to the POU2AF1 family.

    • 翻译后修饰

      Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • B cell Oct binding protein 1 antibody
        • B cell specific coactivator OBF 1 antibody
        • B cell specific coactivator OBF1 antibody
        • B-cell-specific coactivator OBF-1 antibody
        • BOB 1 antibody
        • BOB-1 antibody
        • OBF 1 antibody
        • OBF1 antibody
        • OBF1_HUMAN antibody
        • OCA B antibody
        • OCA-B antibody
        • OCAB antibody
        • OCT binding factor 1 antibody
        • OCT-binding factor 1 antibody
        • POU class 2 associating factor 1 antibody
        • POU domain class 2 associating factor 1 antibody
        • POU domain class 2-associating factor 1 antibody
        • Pou2af1 antibody
        see all

      图片

      • Western blot - Anti-BOB1 antibody (ab3426)
        Western blot - Anti-BOB1 antibody (ab3426)
        ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL). ab3426 at a 1µg/ml concentration staining Bob 1 in mouse spleen lysate (RIPA bufffer, 30 µg total protein per lane, 1 hour incubation) by Western blot (ECL).

      文献 (2)

      发表研究结果有使用 ab3426？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3426 被引用在 2 文献中.

      • Russo MV  et al. Distinct myeloid cell subsets promote meningeal remodeling and vascular repair after mild traumatic brain injury. Nat Immunol 19:442-452 (2018). PubMed: 29662169
      • Joza S  et al. Loss of semaphorin-neuropilin-1 signaling causes dysmorphic vascularization reminiscent of alveolar capillary dysplasia. Am J Pathol 181:2003-17 (2012). PubMed: 23063659

      客户评价及客户问答

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      Question

      Please kindly advise whether the antibody ab3426 anti BOB 1 can be applied on paraffin sections.

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      Answer

      Thank you for your email. To our knowledge, this antibody has yet to be tested in IHC. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will award you 50 points with the Abcam Loyalty Scheme which can be redeemed on a number of rewards. If you have any additional questions, please let us know.

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