Anti-BNIP3L/NIX抗体(ab155010)
Key features and details
- Rabbit polyclonal to BNIP3L/NIX
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-BNIP3L/NIX抗体
参阅全部 BNIP3L/NIX 一抗 -
描述
兔多克隆抗体to BNIP3L/NIX -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Chicken, Cow, Monkey -
免疫原
Synthetic peptide corresponding to Human BNIP3L/NIX aa 95-189.
Database link: O60238 -
阳性对照
- HeLa cells; Molt-4 whole cell lysate; Raji cells; mouse liver whole cell lysate; Human Colon cancer
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab155010于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 24 kDa.
|
|
IHC-P |
1/100 - 1/1000.
|
|
ICC/IF |
1/100 - 1/1000.
|
说明 |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 24 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
-
功能
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. May function as a tumor suppressor. Inhibits apoptosis induced by BNIP3. -
序列相似性
Belongs to the NIP3 family. -
翻译后修饰
Undergoes progressive proteolysis to an 11 kDa C-terminal fragment, which is blocked by the proteasome inhibitor lactacystin. -
细胞定位
Nucleus envelope. Endoplasmic reticulum. Mitochondrion. Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 419522 Chicken
- Entrez Gene: 534615 Cow
- Entrez Gene: 665 Human
- Entrez Gene: 12177 Mouse
- Entrez Gene: 140923 Rat
- Omim: 605368 Human
- SwissProt: Q3T013 Cow
- SwissProt: O60238 Human
see all -
别名
- Adenovirus E1B19k binding protein B5 antibody
- Adenovirus E1B19K-binding protein B5 antibody
- BCL2/adenovirus E1B 19 kd protein interacting protein 3a antibody
see all
图片
-
Anti-BNIP3L/NIX antibody (ab155010) at 1/1000 dilution + Molt4 whole cell lysate at 30 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Anti-BNIP3L/NIX antibody (ab155010) at 1/500 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 24 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded Human colon cancer labeling BNIP3L/NIX with ab155010 antibody at 1/500 dilution.
-
Immunofluorescent analysis of methanol-fixed HeLa cells labeling BNIP3L/NIX with ab155010 antibody at 1/200 dilution. Bottom figure: staining merged with DNA probe (Hoechst 33342).
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab155010 被引用在 1 文献中.
- Duan C et al. Drp1 regulates mitochondrial dysfunction and dysregulated metabolism in ischemic injury via Clec16a-, BAX-, and GSH- pathways. Cell Death Dis 11:251 (2020). PubMed: 32312970