Application
Western blot
Sample
Human Cell lysate - whole cell (MCF-7)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
35 µg
Treatment
20nM siNeg or siBNIP3 for 48 and 72h
Specification
MCF-7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in 1x TBST
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
LI-COR scan
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 55, 20-30 kDa Non-specific: 33 kDa
Positive control
siNeg
Additional data
Additional Notes
Lane1: siNeg 48h, lane2: siBNIP3 48h, lane3: siNeg 72h, lane4: siBNIP3 72h
Used RIPA lysis buffer and nitrocellulose membrane.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 14 2019