Application
Western blot
Sample
Rat Cell lysate - whole cell (neuron)
Gel Running Conditions
Reduced Denaturing (gradient 5-15%)
Loading amount
40 µg
Treatment
10nm to 10´M Arsenic
Specification
neuron
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s)
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (whole molecule)–Peroxidase antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 115 kDa Non-specific: 45, 60, 150 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Jul 11 2017