Anti-BMP2抗体[65529.111] (ab6285)

概述

  • 产品名称
    Anti-BMP2抗体[65529.111]
    参阅全部 BMP2 一抗
  • 描述
    小鼠单克隆抗体[65529.111] to BMP2
  • 宿主
    Mouse
  • 经测试应用
    适用于: WB, ELISA, IHC-P, ICC/IF, Indirect ELISAmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Human
  • 免疫原

    Recombinant full length protein expressed in E. coli (Human).

  • 常规说明
    This product is supplied as 100ug/vial. The concentration range may vary from 0.5mg/ml to 1mg/ml.

性能

应用

Our Abpromise guarantee covers the use of ab6285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 17-18 kDa.
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Do not perform antigen retrieval. PubMed: 17959825
ICC/IF Use a concentration of 1 µg/ml.
Indirect ELISA Use a concentration of 0.5 - 1 µg/ml. To detect a limit of ~3 ng/well recombinant human BMP-2 at 50 and 100 ng/lane under non-reducing and reducing conditions, respectively.

靶标

图片

  • ICC/IF image of ab6285 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6285, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rabbit periosteum labeling BMP2 with ab6285 at 1/100 dilution. The tissue was fixed with paraformaldehyde, followed by blocking in 5% serum for 20 minutes. The tissue was incubated with ab6285 for 12 hours at 4°C in antibody dilution solution. An undiluted polyclonal goat-anti mouse HRP conjugate secondary antibody was used.

    See Abreview

  • ab66285 staining BMP2 in Human small intestine tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1.5% serum for 10 minutes. Antigen retrieval was by heat mediation in citrate (pH6.0). Samples were incubated with primary antibody (1/100 in diluent buffer) for 1 hour. An undiluted HRP-conjugated Goat polyclonal to mouse IgG was used as secondary antibody

    See Abreview

文献

This product has been referenced in:
  • Luo L  et al. Combination treatment of adipose-derived stem cells and adiponectin attenuates pulmonary arterial hypertension in rats by inhibiting pulmonary arterial smooth muscle cell proliferation and regulating the AMPK/BMP/Smad pathway. Int J Mol Med 41:51-60 (2018). Read more (PubMed: 29115380) »
  • Ma YF  et al. Calcium sulfate induced versus PMMA-induced membrane in a critical-sized femoral defect in a rat model. Sci Rep 8:637 (2018). Read more (PubMed: 29330453) »

See all 27 Publications for this product

客户评价及客户问答

Thank you for your enquiry.

We do not have set expiration dates for our products.

Most un-conjugated antibodies are stable and can last for several years if stored properly, so we strongly recommend that you follow the storage inst...

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Thank you for contacting Abcam

The immunogen for ab6285 was recombinant full length protein expressed in E. coli (Human), most likely the pro-BMP2 (= mature BMP2 plus propeptide region attached) form. The exact epitope sequence has not been de...

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Merci de nous avoir contactés.

Nous sommes désolés d'apprendre que le produit que vous avez reçu ne fonctionne pas après quelques mois stocké à -20ºC.
Comme convenu j'ai mis en place...

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Thank you for contacting Abcam.

I have had the opportunity to speak with the lab to try and clarify the statement,"The antibody recognizes recombinant human BMP-2. It shows approximately 25% cross-reactivity with recombinant human BMP-4...

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Merci de nous avoir contactés.

J'ai retrouvé le document que j'avais créé fin d'année 2011 concernant les explications en français pour le stockage des anticorps. Voir pièce jointe.

L...

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Thank you for your inquiry and I apologize that we were not able to receive your earlier phone call.


I have attached the dtatsheets you have requested.


This is where you can find our mouse on mouse staining protocol. Read More

Thank you for your enquiry which has been forwarded to the scientific support team. I am sorry to confirm that because we carry over 70,000 products, it is not feasible for us to keep small sample sizes of our products to send to cusotmers. ...

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I am very pleased to hear you would like to accept our offer and test both antibodies in chicken. The discount codes that I'm using are listed above. Each code will be worth the price you pay for each antibody and can be used with any single future P...

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Thank you for your enquiry. I am happy to confirm that the actual concentration of ab6285 is 1mg/ml. The product is supplied as 100ug/vial. Therefore, the volume you should have in each vial is actually 100ul. If you still have further enquiries...

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Thank you for your enquiry. The human BMP2 protein that this antibody is raised against has only 58% homology with the zebrafish BMP2 protein. As mentioned in the last email, I would suggest this should be over 85% for any chance of detection by the an...

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1-10 of 11 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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