Anti-Bmi1抗体[BMI1/2823] (ab269678)
Key features and details
- Mouse monoclonal [BMI1/2823] to Bmi1
- Suitable for: IHC-P, WB, ICC/IF, Protein Array
- Knockout validated
- Reacts with: Mouse, Human
- Isotype: IgG1
概述
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产品名称
Anti-Bmi1抗体[BMI1/2823]
参阅全部 Bmi1 一抗 -
描述
小鼠单克隆抗体[BMI1/2823] to Bmi1 -
宿主
Mouse -
经测试应用
适用于: IHC-P, WB, ICC/IF, Protein Arraymore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment within Human Bmi1 aa 142-326. The exact sequence is proprietary.
Database link: P35226 -
阳性对照
- WB: MCF7, A431, HEK-293T and NIH/3T3 cell lysate. IHC-P: Human breast carcinoma, colon carcinoma and prostate carcinoma tissue. ICC/IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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纯度
Protein A/G purified -
纯化说明
Purified from bioreactor concentrate. -
克隆
单克隆 -
克隆编号
BMI1/2823 -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Positive Controls
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab269678于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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Protein Array |
Use at an assay dependent concentration.
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说明 |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 36 kDa. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Protein Array
Use at an assay dependent concentration. |
靶标
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功能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2. -
序列相似性
Contains 1 RING-type zinc finger. -
翻译后修饰
Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 100532731 Human
- Entrez Gene: 648 Human
- Entrez Gene: 12151 Mouse
- Omim: 164831 Human
- SwissProt: P35226 Human
- SwissProt: P25916 Mouse
- Unigene: 380403 Human
- Unigene: 289584 Mouse
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别名
- B lymphoma Mo MLV insertion region (mouse) antibody
- B lymphoma Mo MLV insertion region 1 homolog antibody
- Bmi 1 antibody
see all
图片
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All lanes : Anti-Bmi1 antibody [BMI1/2823] (ab269678)
Lane 1 : Wild-type MCF7 cell lysate
Lane 2 : BMI1 knockout MCF7 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 36 kDaLanes 1- 4: Merged signal (red and green). Green - ab269678 observed at 36 kDa. Red - Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) observed at 50 kDa.
ab269678 was shown to react with Bmi1 in wild-type MCF7 cells in western blot. Loss of signal was observed when knockout cell line ab262319 (knockout cell lysate ab256851) was used. Wild-type MCF7 and BMI1 knockout MCF7 cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab269678 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) overnight at 4°C at a 2 µg/ml and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for Bmi1 using ab269678 at 2 μg/ml in immunohistochemical analysis.
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PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Bmi1 using ab269678 at 2 μg/ml in ICC/IF. The secondary antibody is a Goat anti-mouse IgG-CF488 conjugate (Green). The membrane is stained with Phalloidin (Red).
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Anti-Bmi1 antibody [BMI1/2823] (ab269678) at 2 µg/ml + NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Predicted band size: 36 kDa -
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Bmi1 using ab269678 at 2 μg/ml in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Bmi1 using ab269678 at 2 μg/ml in immunohistochemical analysis.
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Analysis of Protein Array containing more than 19,000 full-length human proteins using ab269678.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (3)
ab269678 被引用在 3 文献中.
- Weng J et al. YTHDC1 Promotes Stemness Maintenance and Malignant Progression in Head and Neck Squamous Cell Carcinoma. Stem Cells Int 2022:7494354 (2022). PubMed: 36411870
- Yang G et al. ZNRD1-AS1 knockdown alleviates malignant phenotype of retinoblastoma through miR-128-3p/BMI1 axis. Am J Transl Res 13:5866-5879 (2021). PubMed: 34306331
- Li W et al. MUC1-C drives stemness in progression of colitis to colorectal cancer. JCI Insight 5:N/A (2020). PubMed: 32427590