Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10%)
Sample
Mouse Cell lysate - whole cell (primary hepatoctyes)
Specification
primary hepatoctyes
Blocking step
I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: I-Block buffer
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/15000
Detection
Detection method
ECL
Bands
Specific: 70 kDa Non-specific: 42, 45.60, 72, 130 kDa
Exposure
1 minute(s) and 0 second(s)
Additional data
Abcam response
We would like to thank the user for submitting this abreview. Additional data on our products is always welcome. In this case, the many non-specific bands are unfortunate and our scientific support team is looking into this matter.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 05 2013